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Western Blot (WB)
(MCAF2 Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the MCAF2 antibody detected the MCAF2 protein (arrow).)
Rabbit anti-Human, Mouse MCAF2 Polyclonal Antibody | anti-MCAF2 antibody
MCAF2, ID (ATF7IP2, MCAF2, Activating transcription factor 7-interacting protein 2, MBD1-containing chromatin-associated factor 2) (FITC)
Gene Names
ATF7IP2; MCAF2
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MCAF2; Polyclonal Antibody; ID (ATF7IP2; Activating transcription factor 7-interacting protein 2; MBD1-containing chromatin-associated factor 2) (FITC); ATF7IP2; MBD1-containing chromatin-associated factor 2; anti-MCAF2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-MCAF2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
MCAF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 325-355 amino acids from the Central region of human MCAF2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MCAF2 Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the MCAF2 antibody detected the MCAF2 protein (arrow).)
Western Blot (WB)
(MCAF2 Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the MCAF2 antibody detected the MCAF2 protein (arrow).)
Western Blot (WB)
(MCAF2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MCAF2 antibody detected the MCAF2 protein (arrow).)
Western Blot (WB)
(MCAF2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MCAF2 antibody detected the MCAF2 protein (arrow).)
Immunohistochemistry (IHC)
(MCAF2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCAF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MCAF2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCAF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(MCAF2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MCAF2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-MCAF2 antibody
Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone 'Lys-9' trimethylation activity (Probable).
Product Categories/Family for anti-MCAF2 antibody
References
Adkins, D.E., et al. Mol. Psychiatry (2010). In press: Ichimura, T., et al. J. Biol. Chem. 280(14):13928-13935(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,894 Da
NCBI Official Full Name
activating transcription factor 7-interacting protein 2 isoform 1
NCBI Official Synonym Full Names
activating transcription factor 7 interacting protein 2
NCBI Official Symbol
ATF7IP2
NCBI Official Synonym Symbols
MCAF2
NCBI Protein Information
activating transcription factor 7-interacting protein 2
UniProt Protein Name
Activating transcription factor 7-interacting protein 2
UniProt Gene Name
ATF7IP2
UniProt Synonym Gene Names
MCAF2; ATF7-interacting protein 2
UniProt Entry Name
MCAF2_HUMAN
Research Articles on MCAF2
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Product Notes
The MCAF2 atf7ip2 (Catalog #AAA6319499) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCAF2, ID (ATF7IP2, MCAF2, Activating transcription factor 7-interacting protein 2, MBD1-containing chromatin-associated factor 2) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MCAF2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MCAF2 atf7ip2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCAF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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