Rabbit anti-Human MBD3L2 Polyclonal Antibody | anti-MBD3L2 antibody

MBD3L2, CT (MBD3L2, Methyl-CpG-binding domain protein 3-like 2) (Azide free) (HRP)

Cat. Num. AAA6319291

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MBD3L2; Polyclonal Antibody; CT (MBD3L2; Methyl-CpG-binding domain protein 3-like 2) (Azide free) (HRP); Methyl-CpG-binding domain protein 3-like 2; anti-MBD3L2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MBD3L2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MBD3L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-204 amino acids from the C-terminal region of human MBD3L2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MBD3L2 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the MBD3L2 antibody detected the MBD3L2 protein (arrow).)

Related Product Information for anti-MBD3L2 antibody

This gene encodes a protein that is related to methyl-CpG-binding proteins but lacks the methyl-CpG binding domain. The protein has been found in germ cell tumors and some somatic tissues.

Product Categories/Family for anti-MBD3L2 antibody

Antibodies; Binding Proteins

References

Jin, S.G., et al. J. Biol. Chem. 280(13):12700-12709(2005). Jiang, C.L., et al. Genomics 80(6):621-629(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 157389003
• NCBI GeneID: 125997
• NCBI Accession #: NP_653215.2
• NCBI GenBank Nucleotide #: NM_144614.3
• UniProt Accession #: Q8NHZ7
• Molecular Weight: 22,994 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 3-like 2
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 3-like 2
• NCBI Official Symbol: MBD3L2
• NCBI Protein Information: methyl-CpG-binding domain protein 3-like 2; MBD3-like protein 2
• UniProt Protein Name: Methyl-CpG-binding domain protein 3-like 2
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD3L2
• UniProt Synonym Gene Names: MBD3-like protein 2
• UniProt Entry Name: MB3L2_HUMAN

NCBI Description

This gene encodes a protein that is related to methyl-CpG-binding proteins but lacks the methyl-CpG binding domain. The protein has been found in germ cell tumors and some somatic tissues. [provided by RefSeq, Jul 2008]

Uniprot Description

Tissue specificity: Detected at low levels in several somatic tissues. Highly expressed in the ovarian teratocarcinoma cell line PA-1. Ref.1

Miscellaneous: The MBD3L proteins are encoded by strongly repeated regions of the 19p13 chromosome. The exact number of functional copies is unclear, and some of them may represent pseudogenes.

Sequence similarities: Belongs to the MBD3L family.

Sequence caution: The sequence AAM28154.2 differs from that shown. Reason: Frameshift at position 204.