Rabbit anti-Mouse Mark4 Polyclonal Antibody | anti-Mark4 antibody

Mark4, CT (Mark4, Kiaa1860, MAP/microtubule affinity-regulating kinase 4) (Azide free) (HRP)

Cat. Num. AAA6319082

• Gene Names: Mark4; C79806; Markl1; 2410090P21Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Mark4; Polyclonal Antibody; CT (Mark4; Kiaa1860; MAP/microtubule affinity-regulating kinase 4) (Azide free) (HRP); MAP/microtubule affinity-regulating kinase 4; anti-Mark4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Mark4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Mark4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 605-633 amino acids from the C-terminal region of Mouse Mark4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Mark4 Antibody (C-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Mark4 antibody detected the Mark4 protein (arrow).)

Product Categories/Family for anti-Mark4 antibody

Antibodies; Protein Kinases

References

Schneider, A., et al. J. Neurochem. 88(5):1114-1126(2004). Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003). Okazaki, N., et al. DNA Res. 10(1):35-48(2003). Stryke, D., et al. Nucleic Acids Res. 31(1):278-281(2003). Lee, Y.J., et al. FEBS Lett. 472 (2-3), 230-234 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 26986591
• NCBI GeneID: 232944
• NCBI Accession #: NP_758483.1
• NCBI GenBank Nucleotide #: NM_172279.1
• UniProt Accession #: Q8CIP4; Q80T81
• Molecular Weight: 75,415 Da
• NCBI Official Full Name: MAP/microtubule affinity-regulating kinase 4
• NCBI Official Synonym Full Names: MAP/microtubule affinity regulating kinase 4
• NCBI Official Symbol: Mark4
• NCBI Official Synonym Symbols: C79806; Markl1; 2410090P21Rik
• NCBI Protein Information: MAP/microtubule affinity-regulating kinase 4
• UniProt Protein Name: MAP/microtubule affinity-regulating kinase 4
• Protein Family: MAP/microtubule affinity-regulating kinase
• UniProt Gene Name: Mark4
• UniProt Synonym Gene Names: Kiaa1860

Uniprot Description

Serine/threonine-protein kinase (). Phosphorylates the microtubule-associated protein MAPT (). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (). Required for the initiation of axoneme extension during cilium assembly (). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (). Reduces neuronal cell survival (). Plays a role in energy homeostasis by regulating satiety and metabolic rate (PubMed:22992738). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (PubMed:24989893). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation ().