Rabbit anti-Human MAFA Polyclonal Antibody | anti-MAFA antibody

MAFA, CT (MAFA, Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, Transcription factor RIPE3b1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A) (MaxLight 405)

Cat. Num. AAA6317972

• Gene Names: MAFA; hMafA; RIPE3b1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: MAFA; Polyclonal Antibody; CT (MAFA; Transcription factor MafA; Pancreatic beta-cell-specific transcriptional activator; Transcription factor RIPE3b1; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A) (MaxLight 405); V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; anti-MAFA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAFA
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-MAFA antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MAFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288~318 amino acids from the C-terminal region of human MAFA.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MAFA Antibody (C-term) in WiDr cell line lysates (35ug/lane). MAFA (arrow) was detected using the purified Pab.)

Related Product Information for anti-MAFA antibody

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene.

Product Categories/Family for anti-MAFA antibody

Antibodies; Transcription Factors

References

Kondo, T., et al. Mol. Endocrinol. 23(8):1281-1290(2009). Kaneto, H., et al. Adv. Drug Deliv. Rev. 61 (7-8), 489-496 (2009). Garin, I., et al. Diabet. Med. 26(7):746-748(2009). Vanderford, N.L., et al. Arch. Biochem. Biophys. 480(2):138-142(2008). Martin, C.C., et al. J. Mol. Endocrinol. 41(5):315-328(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 71274111
• NCBI GeneID: 389692
• NCBI Accession #: NP_963883.2
• NCBI GenBank Nucleotide #: NM_201589.3
• UniProt Accession #: Q8NHW3
• Molecular Weight: 36,982 Da
• NCBI Official Full Name: transcription factor MafA
• NCBI Official Synonym Full Names: MAF bZIP transcription factor A
• NCBI Official Symbol: MAFA
• NCBI Official Synonym Symbols: hMafA; RIPE3b1
• NCBI Protein Information: transcription factor MafA
• UniProt Protein Name: Transcription factor MafA
• Protein Family: Transcription factor
• UniProt Gene Name: MAFA
• UniProt Entry Name: MAFA_HUMAN

NCBI Description

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008]

Uniprot Description

MAFA: Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds DNA as a homodimer (Probable). Interacts with NEUROD1, PCAF and PDX1. Up-regulated by glucose. Belongs to the bZIP family. Maf subfamily.

Protein type: Transcription factor; DNA-binding; Tumor suppressor; Oncoprotein

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleus

Molecular Function: DNA binding; protein heterodimerization activity; protein homodimerization activity; transcription factor activity

Biological Process: insulin secretion; nitric oxide mediated signal transduction; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to glucose stimulus; transcription from RNA polymerase II promoter