Rabbit anti-Human LIMA1 Polyclonal Antibody | anti-LIMA1 antibody

LIMA1, NT (LIMA1, EPLIN, SREBP3, LIM domain and actin-binding protein 1, Epithelial protein lost in neoplasm) (MaxLight 490)

Cat. Num. AAA6316114

• Gene Names: LIMA1; EPLIN; LDLCQ8; SREBP3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LIMA1; Polyclonal Antibody; NT (LIMA1; EPLIN; SREBP3; LIM domain and actin-binding protein 1; Epithelial protein lost in neoplasm) (MaxLight 490); Epithelial protein lost in neoplasm; anti-LIMA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
• Sequence Length: 600

• Applicable Applications for anti-LIMA1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: LIMA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-163 amino acids from the N-terminal region of human LIMA1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-LIMA1 antibody

EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles (Maul et al., 2003 [PubMed 12566430]).

Product Categories/Family for anti-LIMA1 antibody

Antibodies; Actin Related Proteins

References

Chircop, M., et al. Cell Cycle 8(5):757-764(2009). Abe, K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):13-19(2008). Jiang, W.G., et al. Mol. Cancer 7, 71 (2008): Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 165905591
• NCBI GeneID: 51474
• NCBI Accession #: NP_001107019.1; NP_001107018.1
• NCBI GenBank Nucleotide #: NP_001107019.1
• UniProt Accession #: Q9UHB6
• NCBI Official Full Name: LIM domain and actin-binding protein 1 isoform 3
• NCBI Official Synonym Full Names: LIM domain and actin binding 1
• NCBI Official Symbol: LIMA1
• NCBI Official Synonym Symbols: EPLIN; LDLCQ8; SREBP3
• NCBI Protein Information: LIM domain and actin-binding protein 1
• UniProt Protein Name: LIM domain and actin-binding protein 1
• Protein Family: LIM domain and actin-binding protein
• UniProt Gene Name: LIMA1
• UniProt Synonym Gene Names: EPLIN; SREBP3
• UniProt Entry Name: LIMA1_HUMAN

NCBI Description

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]

Uniprot Description

eplin: Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: focal adhesion; cytoplasm; plasma membrane; stress fiber; cleavage furrow; actin cytoskeleton

Molecular Function: actin monomer binding; actin filament binding; protein binding; zinc ion binding

Biological Process: actin filament bundle formation; ruffle organization and biogenesis; negative regulation of actin filament depolymerization