Rabbit anti-Human KRT9 Polyclonal Antibody | anti-KRT9 antibody

KRT9, ID (K317) (KRT9, Keratin, type I cytoskeletal 9, Cytokeratin-9, Keratin-9) (MaxLight 490)

Cat. Num. AAA6314838

• Gene Names: KRT9; K9; CK-9; EPPK
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: KRT9; Polyclonal Antibody; ID (K317) (KRT9; Keratin; type I cytoskeletal 9; Cytokeratin-9; Keratin-9) (MaxLight 490); Keratin-9; anti-KRT9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KRT9.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-KRT9 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 302-331 in the central region of human KRT9, UniProt Accession #P35527.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS6004755. KRT9 (arrow) was detected usingMBS6004755.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded skin tissue using MBS6004755 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6004755 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HL-60 cells using MBS6004755 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-KRT9 antibody

Antibodies; Cytokeratin

References

1. Feng, W., Han, W. Eur J Dermatol 18 (4), 387-390 (2008). 2. Zhao, J.J., Zhang, Z.H. Int. J. Dermatol. 47 (6), 634-637 (2008). 3. Torchard, D., Blanchet-Bardon, C. Nat. Genet. 6 (1), 106-110 (1994). 4. Reis, A., Kuster, W. Hum. Genet. 90 (1-2), 113-116 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 55956899
• NCBI GeneID: 3857
• NCBI Accession #: NP_000217.2
• NCBI GenBank Nucleotide #: NP_000217.2
• UniProt Accession #: P35527; O00109; Q0IJ47; Q14665
• Molecular Weight: 62064 MW
• NCBI Official Full Name: keratin, type I cytoskeletal 9
• NCBI Official Synonym Full Names: keratin 9
• NCBI Official Symbol: KRT9
• NCBI Official Synonym Symbols: K9; CK-9; EPPK
• NCBI Protein Information: keratin, type I cytoskeletal 9
• UniProt Protein Name: Keratin, type I cytoskeletal 9
• Protein Family: Keratin
• UniProt Gene Name: KRT9
• UniProt Synonym Gene Names: CK-9; K9

NCBI Description

This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma. [provided by RefSeq, Jul 2008]

Uniprot Description

May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.