Rabbit anti-Human KCNK18 Polyclonal Antibody | anti-KCNK18 antibody

KCNK18, ID (KCNK18, TRESK, TRIK, Potassium channel subfamily K member 18, TWIK-related individual potassium channel, TWIK-related spinal cord potassium channel) (PE)

Cat. Num. AAA6312653

• Gene Names: KCNK18; TRIK; MGR13; TRESK; TRESK2; K2p18.1; TRESK-2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KCNK18; Polyclonal Antibody; ID (KCNK18; TRESK; TRIK; Potassium channel subfamily K member 18; TWIK-related individual potassium channel; TWIK-related spinal cord potassium channel) (PE); TWIK-related spinal cord potassium channel; anti-KCNK18 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-KCNK18 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KCNK18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-197 amino acids from the Central region of human KCNK18.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KCNK18 Antibody (Center) western blot analysis in SiHa,U251,MCF-7 cell line lysates (35ug/lane).This demonstrates the KCNK18 antibody detected the KCNK18 protein (arrow).)

Immunohistochemistry (IHC)

(KCNK18 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KCNK18 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-KCNK18 antibody

Two-pore domain potassium channels, such as KCNK18, give rise to background, or leak, potassium conductance, and they regulate diverse cellular functions by adjusting both the resting membrane potential and excitability. KCNK18 is unique among this family of potassium channels in that its activity is regulated by intracellular calcium (summary by Czirjak and Enyedi, 2006 [PubMed 16569637]).

Product Categories/Family for anti-KCNK18 antibody

Antibodies; Ion Channel

References

Lafreniere, R.G., et al. Nat. Med. 16(10):1157-1160(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Egenberger, B., et al. Biochem. Biophys. Res. Commun. 391(2):1262-1267(2010). Pottosin, I.I., et al. Pflugers Arch. 456(6):1037-1048(2008). Czirjak, G., et al. J. Biol. Chem. 283(23):15672-15680(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 32469495
• NCBI GeneID: 338567
• NCBI Accession #: NP_862823.1
• NCBI GenBank Nucleotide #: NM_181840.1
• UniProt Accession #: Q7Z418; Q5SQQ8
• Molecular Weight: 43,671 Da
• NCBI Official Full Name: potassium channel subfamily K member 18
• NCBI Official Synonym Full Names: potassium two pore domain channel subfamily K member 18
• NCBI Official Symbol: KCNK18
• NCBI Official Synonym Symbols: TRIK; MGR13; TRESK; TRESK2; K2p18.1; TRESK-2
• NCBI Protein Information: potassium channel subfamily K member 18
• UniProt Protein Name: Potassium channel subfamily K member 18
• Protein Family: Potassium channel subfamily
• UniProt Gene Name: KCNK18
• UniProt Synonym Gene Names: TRESK; TRIK
• UniProt Entry Name: KCNKI_HUMAN

NCBI Description

Potassium channels play a role in many cellular processes including maintenance of the action potential, muscle contraction, hormone secretion, osmotic regulation, and ion flow. This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains and the encoded protein functions as an outward rectifying potassium channel. A mutation in this gene has been found to be associated with migraine with aura.[provided by RefSeq, Jan 2011]

Uniprot Description

KCNK18: Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation. Interacts with calcineurin. Interacts with YWHAH, in a phosphorylation-dependent manner. Expressed specifically in dorsal root ganglion and trigeminal ganglion neurons. Detected at low levels in spinal cord. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.

Protein type: Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: calcium-activated potassium channel activity; potassium channel activity; outward rectifier potassium channel activity

Biological Process: synaptic transmission; potassium ion transport

Disease: Migraine, With Or Without Aura, Susceptibility To, 13