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Western Blot (WB) (KCNJ4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KCNJ4 antibody detected the KCNJ4 protein (arrow).)

Rabbit anti-Human KCNJ4 Polyclonal Antibody | anti-KCNJ4 antibody

KCNJ4, NT (KCNJ4, IRK3, Inward rectifier potassium channel 4, HIRK2, HRK1, Hippocampal inward rectifier, Inward rectifier K(+) channel Kir2.3, Potassium channel, inwardly rectifying subfamily J member 4) (APC)

Gene Names
KCNJ4; HIR; HRK1; IRK3; HIRK2; IRK-3; Kir2.3
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
KCNJ4; Polyclonal Antibody; NT (KCNJ4; IRK3; Inward rectifier potassium channel 4; HIRK2; HRK1; Hippocampal inward rectifier; Inward rectifier K(+) channel Kir2.3; Potassium channel; inwardly rectifying subfamily J member 4) (APC); inwardly rectifying subfamily J member 4; anti-KCNJ4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-KCNJ4 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
KCNJ4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human KCNJ4.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KCNJ4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KCNJ4 antibody detected the KCNJ4 protein (arrow).)

Western Blot (WB) (KCNJ4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KCNJ4 antibody detected the KCNJ4 protein (arrow).)
Related Product Information for anti-KCNJ4 antibody
Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Product Categories/Family for anti-KCNJ4 antibody
References
Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010): Yan, X., et al. J. Mol. Biol. 392(4):967-976(2009). He, Y., et al. FEBS Lett. 582(15):2338-2342(2008). Ji, W., et al. Nat. Genet. 40(5):592-599(2008). Ureche, O.N., et al. Cell. Physiol. Biochem. 21 (5-6), 347-356 (2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,500 Da
NCBI Official Full Name
inward rectifier potassium channel 4
NCBI Official Synonym Full Names
potassium voltage-gated channel subfamily J member 4
NCBI Official Symbol
KCNJ4
NCBI Official Synonym Symbols
HIR; HRK1; IRK3; HIRK2; IRK-3; Kir2.3
NCBI Protein Information
inward rectifier potassium channel 4
UniProt Protein Name
Inward rectifier potassium channel 4
UniProt Gene Name
KCNJ4
UniProt Synonym Gene Names
IRK3; HIR; IRK-3
UniProt Entry Name
IRK4_HUMAN

NCBI Description

Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HIR: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, potassium

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: postsynaptic membrane; voltage-gated potassium channel complex; cytoplasmic vesicle membrane; basolateral plasma membrane; plasma membrane; cell junction

Molecular Function: protein binding; inward rectifier potassium channel activity; PDZ domain binding

Biological Process: synaptic transmission; potassium ion import; potassium ion transport

Research Articles on KCNJ4

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Product Notes

The KCNJ4 kcnj4 (Catalog #AAA6312567) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KCNJ4, NT (KCNJ4, IRK3, Inward rectifier potassium channel 4, HIRK2, HRK1, Hippocampal inward rectifier, Inward rectifier K(+) channel Kir2.3, Potassium channel, inwardly rectifying subfamily J member 4) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KCNJ4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KCNJ4 kcnj4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCNJ4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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