Rabbit anti-Human, Mouse K0090 Polyclonal Antibody | anti-K0090 antibody

K0090, CT (EMC1, KIAA0090, ER membrane protein complex subunit 1) (APC)

Cat. Num. AAA6311863

• Gene Names: EMC1; CAVIPMR; KIAA0090
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: K0090; Polyclonal Antibody; CT (EMC1; KIAA0090; ER membrane protein complex subunit 1) (APC); EMC1; ER membrane protein complex subunit 1; anti-K0090 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-K0090 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: K0090 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 873-902 amino acids from the C-terminal region of human K0090.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(K0090 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the K0090 antibody detected the K0090 protein (arrow).)

Western Blot (WB)

(K0090 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the K0090 antibody detected the K0090 protein (arrow).)

Immunohistochemistry (IHC)

(K0090 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the K0090 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(K0090 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-K0090 antibody

Antibodies; KIAA; orf

References

Stein, J.L., et al. Neuroimage 53(3):1160-1174(2010). Bhatti, P., et al. Radiat. Res. 173(2):214-224(2010). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 22095331
• NCBI GeneID: 23065
• NCBI Accession #: NP_055862.1
• NCBI GenBank Nucleotide #: NM_015047.2
• UniProt Accession #: Q8N766; Q14700; Q5TG62; Q63HL0; Q63HL3; Q8NBH8; A8K6F3
• Molecular Weight: 109,421 Da
• NCBI Official Full Name: ER membrane protein complex subunit 1 isoform 1
• NCBI Official Synonym Full Names: ER membrane protein complex subunit 1
• NCBI Official Symbol: EMC1
• NCBI Official Synonym Symbols: CAVIPMR; KIAA0090
• NCBI Protein Information: ER membrane protein complex subunit 1
• UniProt Protein Name: ER membrane protein complex subunit 1
• UniProt Gene Name: EMC1
• UniProt Synonym Gene Names: KIAA0090
• UniProt Entry Name: EMC1_HUMAN

NCBI Description

This gene encodes a single-pass type I transmembrane protein, which is a subunit of the endoplasmic reticulum membrane protein complex (EMC). Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2012]