Rabbit anti-Human ING1 Polyclonal Antibody | anti-ING1 antibody

ING1, NT (ING1, Inhibitor of growth protein 1) (Biotin)

Cat. Num. AAA6310511

• Gene Names: ING1; p33; p47; p33ING1; p24ING1c; p33ING1b; p47ING1a
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ING1; Polyclonal Antibody; NT (ING1; Inhibitor of growth protein 1) (Biotin); Inhibitor of growth protein 1; anti-ING1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ING1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ING1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ING1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ING1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the ING1 antibody detected the ING1 protein (arrow).)

Related Product Information for anti-ING1 antibody

This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.

Product Categories/Family for anti-ING1 antibody

Antibodies; Tumor Suppressors

References

Zhu, Z., et al. Hepatology 49(2):504-512(2009). Sayan, B., et al. Hybridoma (Larchmt) (2009). In press: Tallen, G., et al. Oncol. Res. 18 (2-3), 95-105 (2009): Halaschek-Wiener, J., et al. PLoS ONE 4 (8), E6641 (2009): Han, X., et al. Nat. Cell Biol. 10(11):1333-1340(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 392050742
• NCBI GeneID: 3621
• NCBI Accession #: NP_937862.1; NP_937861.1; NP_005528.3
• NCBI GenBank Nucleotide #: NM_198219
• UniProt Accession #: Q9UK53; O00532; O43658; Q53ZR3; Q5T9G8; Q5T9G9; Q5T9H0; Q5T9H1; Q9H007; Q9HD98; Q9HD99; Q9NS83
• Molecular Weight: ~38 kDa
• NCBI Official Full Name: Homo sapiens inhibitor of growth family, member 1 (ING1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: inhibitor of growth family, member 1
• NCBI Official Symbol: ING1
• NCBI Official Synonym Symbols: p33; p47; p33ING1; p24ING1c; p33ING1b; p47ING1a
• NCBI Protein Information: inhibitor of growth protein 1; growth inhibitor ING1; tumor suppressor ING1; growth inhibitory protein ING1
• UniProt Protein Name: Inhibitor of growth protein 1
• Protein Family: Inhibitor of growth protein
• UniProt Gene Name: ING1
• UniProt Entry Name: ING1_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

ING1: Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene. Defects in ING1 are a cause of head and neck squamous cell carcinomas (HNSCC); also known as squamous cell carcinoma of the head and neck. Belongs to the ING family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: nucleus

Molecular Function: protein binding; zinc ion binding; methylated histone residue binding

Biological Process: negative regulation of cell proliferation; protein import into nucleus; positive regulation of transcription, DNA-dependent; chromatin modification; negative regulation of cell growth; cell cycle

Disease: Squamous Cell Carcinoma, Head And Neck