Rabbit anti-Human HTATIP2 Polyclonal Antibody | anti-HTATIP2 antibody

HTATIP2, NT (HTATIP2, CC3, TIP30, Oxidoreductase HTATIP2, 30kD HIV-1 TAT-interacting protein, HIV-1 TAT-interactive protein 2) (FITC)

Cat. Num. AAA6308785

• Gene Names: HTATIP2; CC3; TIP30; SDR44U1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HTATIP2; Polyclonal Antibody; NT (HTATIP2; CC3; TIP30; Oxidoreductase HTATIP2; 30kD HIV-1 TAT-interacting protein; HIV-1 TAT-interactive protein 2) (FITC); HIV-1 TAT-interactive protein 2; anti-HTATIP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 242

• Applicable Applications for anti-HTATIP2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HTATIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-74 amino acids from the N-terminal region of human HTATIP2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HTATIP2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HTATIP2 antibody detected the HTATIP2 protein (arrow).)

Immunohistochemistry (IHC)

(HTATIP2 Antibody (N-term) (AP14762a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HTATIP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-HTATIP2 antibody

Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.

Product Categories/Family for anti-HTATIP2 antibody

Antibodies; Infectious Disease HIV

References

Chen, X., et al. Dig. Dis. Sci. 55(8):2219-2226(2010). Fong, S., et al. BMC Cell Biol. 11, 23 (2010): Nakahara, J., et al. Expert Opin. Ther. Targets 13(12):1375-1386(2009). Tong, X., et al. Am. J. Pathol. 174(5):1931-1939(2009). Nakahara, J., et al. J. Clin. Invest. 119(1):169-181(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 148728168
• NCBI GeneID: 10553
• NCBI Accession #: NP_001091991.1; NP_001091993.1; NP_001091992.1
• NCBI GenBank Nucleotide #: NP_001091991.1
• UniProt Accession #: Q9BUP3
• NCBI Official Full Name: oxidoreductase HTATIP2 isoform b
• NCBI Official Synonym Full Names: HIV-1 Tat interactive protein 2
• NCBI Official Symbol: HTATIP2
• NCBI Official Synonym Symbols: CC3; TIP30; SDR44U1
• NCBI Protein Information: oxidoreductase HTATIP2
• UniProt Protein Name: Oxidoreductase HTATIP2
• Protein Family: Oxidoreductase
• UniProt Gene Name: HTATIP2
• UniProt Entry Name: HTAI2_HUMAN

Uniprot Description

HTATIP2: Oxidoreductase required for tumor suppression. NAPDH- bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.-; Nuclear envelope; Transcription factor; Apoptosis; Tumor suppressor; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: membrane; cytoplasm; nuclear envelope; nucleus

Molecular Function: protein binding; transcription coactivator activity; oxidoreductase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; viral reproduction; apoptosis; nuclear import; angiogenesis; cell differentiation; regulation of angiogenesis; negative regulation of apoptosis