Rabbit anti-Human HSPH1 Polyclonal Antibody | anti-HSPH1 antibody

HSPH1, ID (HSPH1, HSP105, HSP110, KIAA0201, Heat shock protein 105kD, Antigen NY-CO-25, Heat shock 110kD protein) (MaxLight 550)

Cat. Num. AAA6308778

• Gene Names: HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: HSPH1; Polyclonal Antibody; ID (HSPH1; HSP105; HSP110; KIAA0201; Heat shock protein 105kD; Antigen NY-CO-25; Heat shock 110kD protein) (MaxLight 550); Heat shock 110kD protein; anti-HSPH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-HSPH1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: HSPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 557~586 amino acids from the Center region of human HSPH1.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HSPH1 Antibody (Center) in K562 cell line lysates (35ug/lane). HSPH1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with HSPH1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HSPH1 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HSPH1 antibody

HSPH1 prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. This protein inhibits HSPA8/HSC70 ATPase and chaperone activities.

Product Categories/Family for anti-HSPH1 antibody

Antibodies; Heat Shock Proteins

References

Ishihara K., Yasuda K., Hatayama T.Biochim. Biophys. Acta 1444:138-142(1999). Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., DNA Res. 3:321-329(1996). The MGC Project Team Genome Res. 14:2121-2127(2004). Miyazaki M., Nakatsura T., Yokomine K., Senju S., Monji M., Hosaka S., Cancer Sci. 96:695-705(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 42544159
• NCBI GeneID: 10808
• NCBI Accession #: NP_006635.2
• NCBI GenBank Nucleotide #: NM_006644.2
• UniProt Accession #: Q92598; O95739; Q5TBM6; Q5TBM7; Q5TBM8; Q9UPC4
• Molecular Weight: 96,865 Da
• NCBI Official Full Name: heat shock protein 105 kDa
• NCBI Official Synonym Full Names: heat shock 105kDa/110kDa protein 1
• NCBI Official Symbol: HSPH1
• NCBI Official Synonym Symbols: HSP105; HSP105A; HSP105B; NY-CO-25
• NCBI Protein Information: heat shock protein 105 kDa; antigen NY-CO-25; heat shock 105kD beta; heat shock 105kD alpha; heat shock 110 kDa protein; heat shock 105kDa protein 1
• UniProt Protein Name: Heat shock protein 105 kDa
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPH1
• UniProt Synonym Gene Names: HSP105; HSP110; KIAA0201
• UniProt Entry Name: HS105_HUMAN

Uniprot Description

HSP105: a molecular chaperone of the heat shock protein 70 family. Has ATPase activity. Expressed at especially high levels in mammalian brain and has been shown to suppress apoptosis in neuronal cells and to prevent the aggregation of proteins following heat shock. Known to interact with a variety of proteins including alpha-tubulin and the androgen receptor. Two alternatively spliced isoforms have been described.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; microtubule; cytoplasm; extracellular region; cytosol

Molecular Function: protein binding; ATP binding; alpha-tubulin binding

Biological Process: receptor-mediated endocytosis; positive regulation of NK T cell activation; chaperone cofactor-dependent protein folding; positive regulation of MHC class I biosynthetic process; response to unfolded protein