Rabbit anti-Human PFKFB1 Polyclonal Antibody | anti-PFKFB1 antibody

PFKFB1 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1, 6PF-2-K/Fru-2,6-P2ase 1, PFK/FBPase 1, 6PF-2-K/Fru-2,6-P2ase Liver Isozyme, F6PK, HL2K, MGC116715, MGC116717, PFRX)

Cat. Num. AAA630732

• Gene Names: PFKFB1; F6PK; HL2K; PFRX; MGC116715; MGC116717
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PFKFB1; Polyclonal Antibody; PFKFB1 (6-phosphofructo-2-kinase/fructose-2; 6-biphosphatase 1; 6PF-2-K/Fru-2; 6-P2ase 1; PFK/FBPase 1; 6-P2ase Liver Isozyme; F6PK; HL2K; MGC116715; MGC116717; PFRX); Anti -PFKFB1 (6-phosphofructo-2-kinase/fructose-2; anti-PFKFB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PFKFB1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PFKFB1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the center region of human PFKFB1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of anti-PFKFB1 Antibody (Center) in Jurkat cell line lysates (35ug/lane). PFKFB1(arrow) was detected using the purified Pab.)

Related Product Information for anti-PFKFB1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Product Categories/Family for anti-PFKFB1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 64654084
• NCBI GeneID: 5207
• UniProt Accession #: Q4VBA9; P16118; Q4VBA8
• Molecular Weight: 31,422 Da
• NCBI Official Full Name: PFKFB1 protein
• NCBI Official Synonym Full Names: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
• NCBI Official Symbol: PFKFB1
• NCBI Official Synonym Symbols: F6PK; HL2K; PFRX; MGC116715; MGC116717
• NCBI Protein Information: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1; PFK/FBPase 1; OTTHUMP00000023391; 6PF-2-K/Fru-2,6-P2ase 1; 6PF-2-K/Fru-2,6-P2ASE liver isozyme; fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
• UniProt Protein Name: PFKFB1 protein
• Protein Family: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
• UniProt Gene Name: PFKFB1
• UniProt Entry Name: Q4VBA9_HUMAN

NCBI Description

This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq]

Uniprot Description

PFKFB1: Synthesis and degradation of fructose 2,6-bisphosphate. Homodimer. Liver. Phosphorylation results in inhibition of the kinase activity.

Protein type: EC 2.7.1.105; Carbohydrate Metabolism - fructose and mannose; Phosphatase (non-protein); Kinase, other; EC 3.1.3.46; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 complex; cytosol

Molecular Function: identical protein binding; protein binding; fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; kinase binding; ATP binding

Biological Process: organ regeneration; response to glucagon stimulus; glycolysis; response to cAMP; response to glucocorticoid stimulus; glucose metabolic process; pathogenesis; fructose 2,6-bisphosphate metabolic process; response to insulin stimulus; gluconeogenesis; response to starvation; dephosphorylation; carbohydrate phosphorylation; carbohydrate metabolic process; energy reserve metabolic process; positive regulation of glucokinase activity; fructose metabolic process