Rabbit anti-Human HLA-B Polyclonal Antibody | anti-HLA-B antibody

HLA-B, ID (HLA-B, HLAB, HLA class I histocompatibility antigen, B-7 alpha chain, MHC class I antigen B*7) (APC)

Cat. Num. AAA6307034

• Gene Names: HLA-B; AS; HLAB; SPDA1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HLA-B; Polyclonal Antibody; ID (HLA-B; HLAB; HLA class I histocompatibility antigen; B-7 alpha chain; MHC class I antigen B*7) (APC); MHC class I antigen B*7; anti-HLA-B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-HLA-B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-212 amino acids from the Central region of human HLA-B.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HLA-B Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the HLA-B antibody detected the HLA-B protein (arrow).)

Related Product Information for anti-HLA-B antibody

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

Product Categories/Family for anti-HLA-B antibody

Antibodies; HLA

References

Parsons, M.S., et al. J. Infect. Dis. 202 SUPPL 3, S356-S360 (2010): Brockman, M.A., et al. J. Virol. 84(22):11937-11949(2010). Noble, J.A., et al. Diabetes 59(11):2972-2979(2010). Lipponen, K., et al. Diabetes (2010). In press: Liao, Y.Q., et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi 18(4):1055-1058(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 17986001
• NCBI GeneID: 3106
• NCBI Accession #: NP_005505.2
• NCBI GenBank Nucleotide #: NM_005514.6
• UniProt Accession #: P01889; Q29638; Q29681; Q29854; Q29861; Q31613; Q5SRJ2; Q9GIX1; Q9TP95; P03989; P10319; P18463; P18464; P18465; P30460; P30461; P30462; P30464
• Molecular Weight: 40,460 Da
• NCBI Official Full Name: major histocompatibility complex, class I, B
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, B
• NCBI Official Symbol: HLA-B
• NCBI Official Synonym Symbols: AS; HLAB; SPDA1
• NCBI Protein Information: major histocompatibility complex, class I, B; HLA class I histocompatibility antigen, B alpha chain; MHC Class I HLA heavy chain; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen GN00104; MHC class I antigen H
• UniProt Protein Name: HLA class I histocompatibility antigen, B-7 alpha chain
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-B
• UniProt Synonym Gene Names: HLAB
• UniProt Entry Name: 1B07_HUMAN

NCBI Description

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; integral to plasma membrane; endoplasmic reticulum; plasma membrane; integral to membrane; MHC class I protein complex

Molecular Function: peptide antigen binding; chaperone binding; TAP binding; receptor binding

Biological Process: regulation of immune response; regulation of interleukin-12 production; detection of bacterium; viral reproduction; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; regulation of T cell anergy; regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response