Sheep anti-Human KPNA2 Polyclonal Antibody | anti-KPNA2 antibody

KPNA2 (Karyopherin Subunit alpha 2, Importin Subunit alpha-2, Karyopherin Subunit alpha-2, RAG Cohort Protein 1, RCH1, SRP1, SRP1alpha, SRP1-alpha)

Cat. Num. AAA630700

• Gene Names: KPNA2; QIP2; RCH1; IPOA1; SRP1alpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: KPNA2; Polyclonal Antibody; KPNA2 (Karyopherin Subunit alpha 2; Importin Subunit alpha-2; Karyopherin Subunit alpha-2; RAG Cohort Protein 1; RCH1; SRP1; SRP1alpha; SRP1-alpha); Anti -KPNA2 (Karyopherin Subunit alpha 2; anti-KPNA2 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KPNA2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.

• Applicable Applications for anti-KPNA2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5ug/ml
• Immunogen: Purified, E. coli-derived recombinant human KPNA2 (aa1-132; Accession #P52292).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-KPNA2 antibody

KPNA2 (Karyopherin subunit alpha 2; also SRP1a, importin subunit a2 and RAG1) is a 58-60kD member of the importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, KPNA2 first binds to NBS1, and then to importin b1, which mediates movement through the nuclear pore. Human KPNA2 is 529aa in length. It contains an N-terminal IBB/importin b domain (aa2-60), ten Armadillo repeats that bind "cargo" (aa71-496) and three intervening NLSs. There is one splice variant that shows a deletion of aa72-310, coupled to an 11aa substitution for aa389-406. Over aa1-132, human KPNA2 shares 94% aa identity with mouse KPNA2.

Product Categories/Family for anti-KPNA2 antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 15865453
• NCBI GeneID: 3838
• UniProt Accession #: P52292; Q9BRU5; B9EJD6; Q53YE3; Q6NVW7; Q7Z726
• Molecular Weight: 57,862 Da
• NCBI Official Full Name: karyopherin alpha 2
• NCBI Official Synonym Full Names: karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
• NCBI Official Symbol: KPNA2
• NCBI Official Synonym Symbols: QIP2; RCH1; IPOA1; SRP1alpha
• NCBI Protein Information: importin subunit alpha-2; pendulin; SRP1-alpha; RAG cohort 1; importin alpha 1; importin alpha 2; importin-alpha-P1; RAG cohort protein 1; karyopherin subunit alpha-2
• UniProt Protein Name: Importin subunit alpha-2
• UniProt Gene Name: KPNA2
• UniProt Synonym Gene Names: RCH1; SRP1
• UniProt Entry Name: IMA2_HUMAN

NCBI Description

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq]

Uniprot Description

KPNA2: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the importin alpha family.

Protein type: Karyopherin; Nucleoporin; Nuclear import

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol

Molecular Function: protein binding; histone deacetylase binding; nuclear localization sequence binding; protein transporter activity

Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; regulation of DNA recombination; NLS-bearing substrate import into nucleus; DNA metabolic process