Western Blot (WB)
(HARB1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the HARB1 antibody detected the HARB1 protein (arrow).)
Rabbit anti-Mouse HARB1 Polyclonal Antibody | anti-HARB1 antibody
HARB1, NT (HARBI1, C11orf77, Putative nuclease HARBI1, Harbinger transposase-derived nuclease) (FITC)
Gene Names
HARBI1; C11orf77
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HARB1; Polyclonal Antibody; NT (HARBI1; C11orf77; Putative nuclease HARBI1; Harbinger transposase-derived nuclease) (FITC); HARBI1; Harbinger transposase-derived nuclease; anti-HARB1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-HARB1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
HARB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the N-terminal region of human HARB1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(HARB1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the HARB1 antibody detected the HARB1 protein (arrow).)
Western Blot (WB)
(HARB1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the HARB1 antibody detected the HARB1 protein (arrow).)
Immunohistochemistry (IHC)
(HARB1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HARB1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(HARB1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HARB1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-HARB1 antibody
The specific function of C11ORF77 remains unknown. It is potentially a transposase-derived protein that may have nuclease activity. It does not however have transposase activity. C11ORF77 is detected in brain, eye, nerve tissue, kidney and lung.
Product Categories/Family for anti-HARB1 antibody
References
Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010). Sinzelle, L., et al. Proc. Natl. Acad. Sci. U.S.A. 105(12):4715-4720(2008). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Kapitonov, V.V., et al. DNA Cell Biol. 23(5):311-324(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,146 Da
NCBI Official Full Name
putative nuclease HARBI1
NCBI Official Synonym Full Names
harbinger transposase derived 1
NCBI Official Symbol
HARBI1
NCBI Official Synonym Symbols
C11orf77
NCBI Protein Information
putative nuclease HARBI1
UniProt Protein Name
Putative nuclease HARBI1
UniProt Gene Name
HARBI1
UniProt Synonym Gene Names
C11orf77
Uniprot Description
HARBI1: Transposase-derived protein that may have nuclease activity (Potential). Does not have transposase activity. Belongs to the HARBI1 family.
Protein type: EC 3.1.-.-; Hydrolase
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: cytosol; microtubule organizing center; nucleus; plasma membrane
Molecular Function: metal ion binding; nuclease activity
Research Articles on HARB1
Similar Products
Product Notes
The HARB1 harbi1 (Catalog #AAA6305430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HARB1, NT (HARBI1, C11orf77, Putative nuclease HARBI1, Harbinger transposase-derived nuclease) (FITC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HARB1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HARB1 harbi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HARB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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