Western Blot (WB)
(H2AFJ Antibody (N-term) western blot analysis in Jurkat,MDA-MB435,MCF-7,Hela cell line lysates (35ug/lane).This demonstrates the H2AFJ antibody detected the H2AFJ protein (arrow).)
Rabbit anti-Human H2AFJ Polyclonal Antibody | anti-H2AFJ antibody
H2AFJ, NT (H2AFJ, Histone H2A.J) (PE)
Gene Names
H2AFJ; H2AJ
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
H2AFJ; Polyclonal Antibody; NT (H2AFJ; Histone H2A.J) (PE); Histone H2A.J; anti-H2AFJ antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-H2AFJ antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
H2AFJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFJ.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(H2AFJ Antibody (N-term) western blot analysis in Jurkat,MDA-MB435,MCF-7,Hela cell line lysates (35ug/lane).This demonstrates the H2AFJ antibody detected the H2AFJ protein (arrow).)
Western Blot (WB)
(H2AFJ Antibody (N-term) western blot analysis in Jurkat,MDA-MB435,MCF-7,Hela cell line lysates (35ug/lane).This demonstrates the H2AFJ antibody detected the H2AFJ protein (arrow).)
Related Product Information for anti-H2AFJ antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Product Categories/Family for anti-H2AFJ antibody
References
Yao, J., et al. Cancer Res. 66(8):4065-4078(2006). de Wit, N.J., et al. Br. J. Cancer 92(12):2249-2261(2005). Chadwick, B.P., et al. Hum. Mol. Genet. 10(10):1101-1113(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,110 Da
NCBI Official Full Name
histone H2A.J
NCBI Official Synonym Full Names
H2A histone family, member J
NCBI Official Symbol
H2AFJ
NCBI Official Synonym Symbols
H2AJ
NCBI Protein Information
histone H2A.J; H2a/j; buforin I
UniProt Protein Name
Histone H2A.J
Protein Family
UniProt Gene Name
H2AFJ
UniProt Synonym Gene Names
H2a/j
UniProt Entry Name
H2AJ_HUMAN
Uniprot Description
H2AFJ: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H2A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 12p12.3
Cellular Component: nucleosome; nucleus
Molecular Function: DNA binding; protein heterodimerization activity
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Product Notes
The H2AFJ h2afj (Catalog #AAA6305228) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The H2AFJ, NT (H2AFJ, Histone H2A.J) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's H2AFJ can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the H2AFJ h2afj for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H2AFJ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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