Rabbit anti-Human GORASP2 Polyclonal Antibody | anti-GORASP2 antibody

GORASP2, ID (GORASP2, GOLPH6, Golgi reassembly-stacking protein 2, Golgi phosphoprotein 6, Golgi reassembly-stacking protein of 55kD, p59) (Azide free) (HRP)

Cat. Num. AAA6303308

• Gene Names: GORASP2; p59; GRS2; GOLPH6; GRASP55
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GORASP2; Polyclonal Antibody; ID (GORASP2; GOLPH6; Golgi reassembly-stacking protein 2; Golgi phosphoprotein 6; Golgi reassembly-stacking protein of 55kD; p59) (Azide free) (HRP); p59; anti-GORASP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 452

• Applicable Applications for anti-GORASP2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GORASP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-214 amino acids from the Central region of human GORASP2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GORASP2 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GORASP2 antibody detected the GORASP2 protein (arrow).)

Related Product Information for anti-GORASP2 antibody

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in establishing the stacked structure of the Golgi apparatus.

Product Categories/Family for anti-GORASP2 antibody

Antibodies; Golgi Proteins

References

Sebastiani, P., et al. Science (2010). In press: Roghi, C., et al. FEBS J. 277(15):3158-3175(2010). Xiang, Y., et al. J. Cell Biol. 188(2):237-251(2010). D'Angelo, G., et al. J. Biol. Chem. 284(50):34849-34860(2009). Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 29826294
• NCBI GeneID: 26003
• NCBI Accession #: NP_056345.3
• NCBI GenBank Nucleotide #: NP_056345.3
• UniProt Accession #: Q9H8Y8
• NCBI Official Full Name: Golgi reassembly-stacking protein 2 isoform 1
• NCBI Official Synonym Full Names: golgi reassembly stacking protein 2
• NCBI Official Symbol: GORASP2
• NCBI Official Synonym Symbols: p59; GRS2; GOLPH6; GRASP55
• NCBI Protein Information: Golgi reassembly-stacking protein 2
• UniProt Protein Name: Golgi reassembly-stacking protein 2
• Protein Family: Golgi reassembly-stacking protein
• UniProt Gene Name: GORASP2
• UniProt Synonym Gene Names: GOLPH6; GRS2; GOLPH6; GRASP55
• UniProt Entry Name: GORS2_HUMAN

NCBI Description

This gene encodes a member of the Golgi reassembly stacking protein family. These proteins may play a role in the stacking of Golgi cisternae and Golgi ribbon formation, as well as Golgi fragmentation during apoptosis or mitosis. The encoded protein also plays a role in the intracellular transport of transforming growth factor alpha and may function as a molecular chaperone. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

GRASP55: a golgi reassembly-stacking protein (GRASP) protein involved in the stacking of Golgi cisternae. May be involved in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as the TGF-alpha receptor. Oligomerization of its N-terminal GRASP domain regulates the formation of Golgi stacks, a process that is regulated by phosphorylation within the C-terminal Ser/Pro-rich domain. Forms a RAB2 effector complex with BLZF1 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of the TGF-alpha receptor, prior its transit in the trans-Golgi. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: Golgi membrane; Golgi apparatus; membrane

Molecular Function: protein binding

Biological Process: organelle organization and biogenesis; mitotic cell cycle