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Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS7601801 (NBR1 antibody) at dilution of 1:100)

Rabbit NBR1 Polyclonal Antibody | anti-NBR1 antibody

NBR1 Rabbit Polyclonal

Reactivity
Human, Mouse, Rat, Monkey
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Synonyms
NBR1; Polyclonal Antibody; NBR1 Rabbit Polyclonal; anti-NBR1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Form/Format
Liquid
Sequence Length
988
Applicable Applications for anti-NBR1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Immunogen
Neighbor of BRCA1 gene 1
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS7601801 (NBR1 antibody) at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS7601801 (NBR1 antibody) at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells, using NBR1 antibody MBS7601801 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red) .)

Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells, using NBR1 antibody MBS7601801 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red) .)

Immunoprecipitation (IP)

(IP Result of anti-NBR1 (IP: MBS7601801, 4ug; Detection:1 MBS7601801 1:800) with HeLa cells lysate 2500ug.)

Immunoprecipitation (IP) (IP Result of anti-NBR1 (IP: MBS7601801, 4ug; Detection:1 MBS7601801 1:800) with HeLa cells lysate 2500ug.)

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7601801 (NBR1 antibody) at dilution of 1:1000)

Western Blot (WB) (HeLa cells were subjected to SDS PAGE followed by western blot with MBS7601801 (NBR1 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
109,958 Da
NCBI Official Full Name
Nbr1
UniProt Protein Name
Next to BRCA1 gene 1 protein
UniProt Gene Name
Nbr1
UniProt Synonym Gene Names
M17s2
UniProt Entry Name
NBR1_MOUSE

Uniprot Description

NBR1: Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. Homooligomer and heterooligomer. Interacts with TRIM55. Interacts with SQSTM1, titin/TTN, RNF29, USP8, SQSTM1, MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2. Binds to ubiquitin and ubiquitinated proteins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Cellular Component: autophagic vacuole; cytoplasm; cytoplasmic vesicle; cytosol; late endosome; lysosome; membrane; mitochondrion

Molecular Function: metal ion binding; mitogen-activated protein kinase binding; protein binding; ubiquitin binding; zinc ion binding

Biological Process: macroautophagy; negative regulation of osteoblast differentiation; protein oligomerization; regulation of bone mineralization; regulation of stress-activated MAPK cascade

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Product Notes

The NBR1 nbr1 (Catalog #AAA7601801) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NBR1 Rabbit Polyclonal reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's NBR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the NBR1 nbr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NBR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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