Rabbit anti-Human Caveolin 1 Polyclonal Antibody | anti-CAV1 antibody

Caveolin 1, phosphorylated (Tyr14) (CAV1, caveolae protein, 22kD, HGNC:1527, CAV, MSTP085, VIP21, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32)

Cat. Num. AAA630202

• Gene Names: CAV1; CGL3; BSCL3; VIP21; MSTP085
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: Caveolin 1; Polyclonal Antibody; phosphorylated (Tyr14) (CAV1; caveolae protein; 22kD; HGNC:1527; CAV; MSTP085; VIP21; caveolin 1; alpha isoform; beta isoform; cell growth-inhibiting protein 32); Anti -Caveolin 1; anti-CAV1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of caveolin-1 phosphorylated at tyrosine 14. Does not crossreact with caveolin-2, -3 or caveolin-1beta, the short isoform of caveolin-1.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100 ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-CAV1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr14 of human caveolin-1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blotting analysis of extracts from HeLa cells induced with H2O2 for indicated times, using Phospho-Caveolin-1 (Tyr14) (upper) and caveolin-1 (lower).)

Related Product Information for anti-CAV1 antibody

The 21-24kD integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of caveolins (caveolin-1, -2 and -3) have been identified with different tissue distributions. Caveolins form hetero-and homooligomers, which interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis. It is also implicated in neurodegenerative disease (2). Caveolins also interact with multiple signaling molecules, such as G-alpha subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Tyrosine 14 has been identified as one of the tyrosine phosphorylation sites on caveolin-1 (3,4). Phosphorylation at Tyr14 is essential for its association with SH2 or PTB domaincontaining adaptor proteins, such as GRB7 (5). Phosphorylation at serine 80 is a key regulatory event for its binding to the ER membrane and entry into the secretory pathway (6).

Product Categories/Family for anti-CAV1 antibody

Antibodies; Abs to Membrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4972627
• NCBI GeneID: 857
• UniProt Accession #: Q03135; Q9UGP1; Q9UNG1; Q9UQH6; Q2TNI1; Q59E85; Q71VB8; Q7Z4F3
• Molecular Weight: 20,472 Da
• NCBI Official Full Name: caveolin 1
• NCBI Official Synonym Full Names: caveolin 1, caveolae protein, 22kDa
• NCBI Official Symbol: CAV1
• NCBI Official Synonym Symbols: CGL3; BSCL3; VIP21; MSTP085
• NCBI Protein Information: caveolin-1; OTTHUMP00000025031; OTTHUMP00000195988; OTTHUMP00000195989; OTTHUMP00000196475; OTTHUMP00000198308; cell growth-inhibiting protein 32
• UniProt Protein Name: Caveolin-1
• Protein Family: Caveolin
• UniProt Gene Name: CAV1
• UniProt Synonym Gene Names: CAV
• UniProt Entry Name: CAV1_HUMAN

NCBI Description

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.

Uniprot Description

Caveolin-1: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N- terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK. Expressed in muscle and lung, less so in liver, brain and kidney. Belongs to the caveolin family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q31.1

Cellular Component: protein complex; focal adhesion; basolateral plasma membrane; integral to plasma membrane; endoplasmic reticulum; lipid particle; cell cortex; caveola; acrosomal membrane; lipid raft; cilium; Golgi membrane; early endosome membrane; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; intracellular; cytoplasmic vesicle; endosome

Molecular Function: identical protein binding; protein binding; protease activator activity; enzyme binding; cholesterol binding; patched binding; protein complex scaffold; nitric-oxide synthase binding; structural molecule activity; protein kinase binding; receptor binding

Biological Process: mammary gland involution; viral reproduction; negative regulation of epithelial cell differentiation; negative regulation of tyrosine phosphorylation of Stat5 protein; nitric oxide homeostasis; negative regulation of BMP signaling pathway; calcium ion homeostasis; protein localization; sequestering of lipid; regulation of the force of heart contraction by chemical signal; regulation of fatty acid metabolic process; negative regulation of protein binding; inactivation of MAPK activity; regulation of smooth muscle contraction; maintenance of cellular protein localization; skeletal muscle development; cytosolic calcium ion homeostasis; negative regulation of nitric-oxide synthase activity; cellular response to starvation; membrane depolarization; cholesterol homeostasis; response to estrogen stimulus; T cell costimulation; negative regulation of endothelial cell proliferation; negative regulation of protein ubiquitination; regulation of nitric-oxide synthase activity; response to calcium ion; response to progesterone stimulus; leukocyte migration; negative regulation of JAK-STAT cascade; lactation; vesicle organization and biogenesis; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of pinocytosis; nitric oxide metabolic process; mammary gland development; calcium ion transport; negative regulation of cytokine and chemokine mediated signaling pathway; angiogenesis; vasculogenesis; protein homooligomerization; vasoconstriction; cholesterol transport; negative regulation of MAPKKK cascade; negative regulation of nitric oxide biosynthetic process; MAPKKK cascade; positive regulation of metalloenzyme activity; positive regulation of peptidyl-serine phosphorylation; regulation of peptidase activity; cellular calcium ion homeostasis; triacylglycerol metabolic process; regulation of blood coagulation; response to hypoxia; positive regulation of vasoconstriction; blood coagulation; vascular endothelial growth factor receptor signaling pathway

Disease: Lipodystrophy, Congenital Generalized, Type 3; Partial Lipodystrophy, Congenital Cataracts, And Neurodegeneration Syndrome; Pulmonary Hypertension, Primary, 3