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Rabbit GATA1 Polyclonal Antibody | anti-GATA1 antibody

GATA1, phosphorylated (Ser142) (Erythroid Transcription Factor, Eryf1, GATA-binding Factor 1, GATA-1, GF-1, NF-E1 DNA-binding Protein, ERYF1, GF1)

Gene Names
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; GATA-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
GATA1; Polyclonal Antibody; phosphorylated (Ser142) (Erythroid Transcription Factor; Eryf1; GATA-binding Factor 1; GATA-1; GF-1; NF-E1 DNA-binding Protein; ERYF1; GF1); Anti -GATA1; anti-GATA1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GATA1 when phosphorylated at serine 142. Species Cross-Reactivity: mouse, rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-GATA1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry.
Dilution: Immunofluorescence: 1:100-1:200
Western Blot: 1:500-1:1,000
Immunohistochemistry: 1:50-1:100
Immunogen
Synthetic phosphopeptide corresponding to human GATA-1 around the phosphorylation site of serine 142 (R-L-SP-P-D).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-GATA1 antibody
Globin transcription factor 1 (GATA-1) is a transcription activator belonging to the GATA family of transcription factors with importance in cell growth. GATA-1 is a Cys2-Cys2 zinc finger DNA binding protein commonly found in erthroid (red blood cell) and emagkaryotic (platelet producing cell) and it is critical in the development process of both. It binds to the consensus sequence of [AT]GATA[AG] within regulatory regions of globin genes and genes in erythroid cells, leading to transcription of alpha spectrin structural protein necessary for red blood cell's shape. In human, GATA-1 can enhance transcription rate up to 100 times. Mutation to GATA-1 encoding gene has been implicated to X-linked dyserythropoietic anemia, thrombocytopenia, Down's syndrome in children, congenital transient myeloproliferative syndrome, and acute megakaryocytic leukemia.
Product Categories/Family for anti-GATA1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
42,751 Da
NCBI Official Full Name
GATA1 protein
NCBI Official Synonym Full Names
GATA binding protein 1 (globin transcription factor 1)
NCBI Official Symbol
GATA1
NCBI Official Synonym Symbols
GF1; GF-1; NFE1; XLTT; ERYF1; GATA-1
NCBI Protein Information
erythroid transcription factor; OTTHUMP00000023259; OTTHUMP00000023260; GATA-binding factor 1; NF-E1 DNA-binding protein; transcription factor GATA1; globin transcription factor 1; erythroid transcription factor 1
UniProt Protein Name
Erythroid transcription factor
Protein Family
UniProt Gene Name
GATA1
UniProt Synonym Gene Names
ERYF1; GF1
UniProt Entry Name
GATA1_HUMAN

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq]

Uniprot Description

GATA1: Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with EP300. Erythrocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; p53 binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity; DNA bending activity

Biological Process: transcription from RNA polymerase II promoter; erythrocyte development; megakaryocyte differentiation; embryonic hemopoiesis; in utero embryonic development; positive regulation of erythrocyte differentiation; eosinophil differentiation; male gonad development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of bone mineralization; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of osteoblast proliferation; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; platelet formation; blood coagulation; basophil differentiation; negative regulation of apoptosis

Disease: Thrombocytopenia With Beta-thalassemia, X-linked; Down Syndrome; Thrombocytopenia, X-linked, With Or Without Dyserythropoietic Anemia; Anemia, X-linked, With Or Without Neutropenia And/or Platelet Abnormalities

Research Articles on GATA1

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Product Notes

The GATA1 gata1 (Catalog #AAA630140) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GATA1, phosphorylated (Ser142) (Erythroid Transcription Factor, Eryf1, GATA-binding Factor 1, GATA-1, GF-1, NF-E1 DNA-binding Protein, ERYF1, GF1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GATA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Dilution: Immunofluorescence: 1:100-1:200 Western Blot: 1:500-1:1,000 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the GATA1 gata1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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