Rabbit anti-Human, Mouse GAS1 Polyclonal Antibody | anti-GAS1 antibody

GAS1, NT (GAS1, Growth arrest-specific protein 1) (APC)

Cat. Num. AAA6301259

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry
• Purity: Purified by protein A/G affinity chromatography
• Synonyms: GAS1; Polyclonal Antibody; NT (GAS1; Growth arrest-specific protein 1) (APC); Growth arrest-specific protein 1; anti-GAS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GAS1.
• Purity/Purification: Purified by protein A/G affinity chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-GAS1 antibody: Suitable for use in FLISA, Western Blot (WB), Immunofluorescence (IF) and Immunohistochemisty (IHC).; Other potential application, though not tested is Direct Flow Cytometry. Other applications not tested.
• Application Notes: Immunohistochemistry: Formalin-fixed, paraffin-embedded sections.; Optimal dilutions to be determined by the researcher.; ; Note: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino-acid 95-127 in the N-Terminal region of human GAS1
• Species Crossreactivity: Mouse
• Grade: Affinity Purified
• Preparation and Storage: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.; Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NCI-H460 cell line lysates (35ug/lane) using MBS6301259.This demonstrates that MBS6301259 detected the GAS1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue tissue using MBS6301259 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS6301259 for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NCI-H460 cells using MBS6301259 followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-GAS1 antibody

Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.

Product Categories/Family for anti-GAS1 antibody

Antibodies; Proteins

References

Ribeiro, L.A., et al. Am. J. Med. Genet. A 152A (7), 1688-1694 (2010): Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010): Rittie, L., et al. Aging Cell 8(6):738-751(2009). Zhao, L., et al. J. Biol. Chem. 284(39):26273-26285(2009). Dominguez-Monzon, G., et al. Int. J. Dev. Neurosci. 27(4):305-313(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 167466169
• NCBI GeneID: 2619
• NCBI Accession #: NP_002039
• NCBI GenBank Nucleotide #: NM_002048
• UniProt Accession #: P54826; Q6B086; B9EGM4
• NCBI Official Full Name: growth arrest-specific protein 1
• NCBI Official Synonym Full Names: growth arrest-specific 1
• NCBI Official Symbol: GAS1
• NCBI Protein Information: growth arrest-specific protein 1; GAS-1; Growth arrest-specific gene-1
• UniProt Protein Name: Growth arrest-specific protein 1
• Protein Family: Germacrene A synthase
• UniProt Gene Name: GAS1
• UniProt Synonym Gene Names: GAS-1
• UniProt Entry Name: GAS1_HUMAN

Uniprot Description

GAS1: Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 9q21.3-q22

Cellular Component: anchored to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: developmental growth; axon guidance; cell fate commitment; camera-type eye development; outer ear morphogenesis; programmed cell death; cerebellum morphogenesis; palate development; embryonic cranial skeleton morphogenesis; middle ear morphogenesis; odontogenesis; regulation of apoptosis; negative regulation of smoothened signaling pathway; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; negative regulation of mitotic cell cycle; embryonic digit morphogenesis; negative regulation of cell growth; cell cycle arrest; positive regulation of epithelial cell proliferation; dorsoventral neural tube patterning; regulation of smoothened signaling pathway; negative regulation of epithelial cell proliferation; eye morphogenesis