Western Blot (WB)
(GAGE2B Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GAGE2B antibody detected the GAGE2B protein (arrow).)
Rabbit anti-Human GAGE2B Polyclonal Antibody | anti-GAGE2B antibody
GAGE2B, NT (GAGE2B, GAGE2, G antigen 2B/2C, Cancer/testis antigen 4.2, G antigen 2C) (Azide free) (HRP)
Gene Names
GAGE2C; CT4.2; GAGE2; GAGE-2; GAGE-2C
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GAGE2B; Polyclonal Antibody; NT (GAGE2B; GAGE2; G antigen 2B/2C; Cancer/testis antigen 4.2; G antigen 2C) (Azide free) (HRP); G antigen 2C; anti-GAGE2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Sequence Length
116
Applicable Applications for anti-GAGE2B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
GAGE2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GAGE2B.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(GAGE2B Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GAGE2B antibody detected the GAGE2B protein (arrow).)
Western Blot (WB)
(GAGE2B Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GAGE2B antibody detected the GAGE2B protein (arrow).)
Related Product Information for anti-GAGE2B antibody
This gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The antigenic peptide YRPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes. [provided by RefSeq].
Product Categories/Family for anti-GAGE2B antibody
References
Sun, F., et al. Mol. Cancer Ther. 8(12):3191-3202(2009). Gjerstorff, M.F., et al. Tissue Antigens 71(3):187-192(2008). Ross, M.T., et al. Nature 434(7031):325-337(2005). De Backer, O., et al. Cancer Res. 59(13):3157-3165(1999). Scarcella, D.L., et al. Clin. Cancer Res. 5(2):335-341(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
G antigen 2B/2C
NCBI Official Synonym Full Names
G antigen 2C
NCBI Official Symbol
GAGE2C
NCBI Official Synonym Symbols
CT4.2; GAGE2; GAGE-2; GAGE-2C
NCBI Protein Information
G antigen 2B/2C
UniProt Protein Name
G antigen 2B/2C
Protein Family
UniProt Gene Name
GAGE2B
UniProt Synonym Gene Names
GAGE2; GAGE-2B; GAGE-2C; CT4.2
UniProt Entry Name
GAG2B_HUMAN
NCBI Description
This gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The antigenic peptide YRPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes. [provided by RefSeq, Jul 2008]
Uniprot Description
GAGE2B/C: Antigen, recognized on melanoma by autologous cytolytic T-lymphocytes. Belongs to the GAGE family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: Xp11.23
Research Articles on GAGE2B
Similar Products
Product Notes
The GAGE2B gage2b (Catalog #AAA6301020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAGE2B, NT (GAGE2B, GAGE2, G antigen 2B/2C, Cancer/testis antigen 4.2, G antigen 2C) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAGE2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GAGE2B gage2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAGE2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
