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Western Blot (WB) (Western Blot analysis in CEM cell line lysates, using MBS641948 (35ug/lane). This demonstrates that MBS641948 detected the FSTL3 protein (arrow).)

Rabbit anti-Human FSTL3 Polyclonal Antibody | anti-FSTL3 antibody

FSTL3, CT (FSTL3, FLRG, Follistatin-related protein 3, Follistatin-like protein 3, Follistatin-related gene protein) (APC)

Gene Names
FSTL3; FLRG; FSRP
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
FSTL3; Polyclonal Antibody; CT (FSTL3; FLRG; Follistatin-related protein 3; Follistatin-like protein 3; Follistatin-related gene protein) (APC); Follistatin-related gene protein; anti-FSTL3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human FSTL3.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-FSTL3 antibody
Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 229-258 in the C-terminal region of human FSTL3, Swiss/UniProt Accession: O95633.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates, using MBS641948 (35ug/lane). This demonstrates that MBS641948 detected the FSTL3 protein (arrow).)

Western Blot (WB) (Western Blot analysis in CEM cell line lysates, using MBS641948 (35ug/lane). This demonstrates that MBS641948 detected the FSTL3 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in HepG2 whole cell lysate (20ug) using MBS641948 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 28kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Western Blot analysis in HepG2 whole cell lysate (20ug) using MBS641948 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 28kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot analysis in A431 whole cell lysate (20ug) using MBS641948 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 28kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Western Blot analysis in A431 whole cell lysate (20ug) using MBS641948 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 28kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry staining FSTL3 in human testis tissue sectionsusingMBS641948. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hrs at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with MBS641948 (1:25) for 1 hr at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry staining FSTL3 in human testis tissue sectionsusingMBS641948. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hrs at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with MBS641948 (1:25) for 1 hr at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Product Categories/Family for anti-FSTL3 antibody
References
1. Miron, P., et al. Prenat. Diagn. 30(3):224-228 (2010). 2. Thadhani, R., et al. Diabetes Care 33(3):664-669 (2010). 3. Bloise, E., et al. BMC Cancer 9, 320 (2009). 4. Stamler, R., et al. J. Biol. Chem. 283(47):32831-32838 (2008). 5. Lara-Pezzi, E., et al. Endocrinology 149(11):5822-5827 (2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,960 Da
NCBI Official Full Name
follistatin-related protein 3
NCBI Official Synonym Full Names
follistatin like 3
NCBI Official Symbol
FSTL3
NCBI Official Synonym Symbols
FLRG; FSRP
NCBI Protein Information
follistatin-related protein 3
UniProt Protein Name
Follistatin-related protein 3
UniProt Gene Name
FSTL3
UniProt Synonym Gene Names
FLRG

NCBI Description

Follistatin-like 3 is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10.

Research Articles on FSTL3

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Product Notes

The FSTL3 fstl3 (Catalog #AAA6300247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FSTL3, CT (FSTL3, FLRG, Follistatin-related protein 3, Follistatin-like protein 3, Follistatin-related gene protein) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FSTL3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the FSTL3 fstl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FSTL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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