Rabbit anti-Human, Mouse Ephb6 Polyclonal Antibody | anti-Ephb6 antibody

Ephb6, ID (Ephb6, Cekl, Ephrin type-B receptor 6, MEP, Tyrosine-protein kinase-defective receptor EPH-6) (Azide free) (HRP)

Cat. Num. AAA6296067

• Gene Names: Ephb6; Mep; Cekl
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Ephb6; Polyclonal Antibody; ID (Ephb6; Cekl; Ephrin type-B receptor 6; MEP; Tyrosine-protein kinase-defective receptor EPH-6) (Azide free) (HRP); Tyrosine-protein kinase-defective receptor EPH-6; anti-Ephb6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Ephb6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Ephb6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 502-530 amino acids from the Central region of mouse Ephb6.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Ephb6 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Mouse Ephb6 antibody detected the Mouse Ephb6 protein (arrow).)

Related Product Information for anti-Ephb6 antibody

Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2 (By similarity).

Product Categories/Family for anti-Ephb6 antibody

Antibodies; Protein Kinases

References

Islam, S., et al. Dig. Dis. Sci. 55(9):2478-2488(2010). Lieben, L., et al. Bone 47(2):301-308(2010). Mordan-McCombs, S., et al. J. Steroid Biochem. Mol. Biol. 121 (1-2), 368-371 (2010): Zirzow, S., et al. Dev. Biol. 336(2):145-155(2009). Chou, S.J., et al. Nat. Neurosci. 12(11):1381-1389(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 226437671
• NCBI GeneID: 13848
• NCBI Accession #: NP_001139823.1
• NCBI GenBank Nucleotide #: NM_001146351.1
• UniProt Accession #: O08644; Q3TQ77; Q8BN76; Q8K0A9
• Molecular Weight: 110,107 Da
• NCBI Official Full Name: ephrin type-B receptor 6
• NCBI Official Synonym Full Names: Eph receptor B6
• NCBI Official Symbol: Ephb6
• NCBI Official Synonym Symbols: Mep; Cekl
• NCBI Protein Information: ephrin type-B receptor 6; ephrin type A receptor; chicken eph/elk receptor-like protein; tyrosine-protein kinase-defective receptor EPH-6
• UniProt Protein Name: Ephrin type-B receptor 6
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: Ephb6
• UniProt Synonym Gene Names: Cekl
• UniProt Entry Name: EPHB6_MOUSE

Uniprot Description

Function: Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2

By similarity.

Subunit structure: Interacts with CBL and EPHB1. Interacts with FYN; this interaction takes place in a ligand-independent manner

By similarity.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted.

Tissue specificity: High level in thymus, and brain. Very low levels of expression in kidney, lung, liver, bone marrow, skeletal muscle, spleen from 2 week old and adult mice, heart, testes and embryonic stem cells. Ref.1

Domain: The protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2

By similarity.

Post-translational modification: Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.Contains 1 Eph LBD (Eph ligand-binding) domain.Contains 2 fibronectin type-III domains.Contains 1 protein kinase domain.Contains 1 SAM (sterile alpha motif) domain.