Rabbit anti-Human ELL3 Polyclonal Antibody | anti-ELL3 antibody

ELL3, CT (ELL3, RNA polymerase II elongation factor ELL3) (Azide free) (HRP)

Cat. Num. AAA6295285

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ELL3; Polyclonal Antibody; CT (ELL3; RNA polymerase II elongation factor ELL3) (Azide free) (HRP); RNA polymerase II elongation factor ELL3; anti-ELL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ELL3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ELL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-360 amino acids from the C-terminal region of human ELL3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ELL3 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ELL3 antibody detected the ELL3 protein (arrow).)

Related Product Information for anti-ELL3 antibody

ELL3 is a elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.

Product Categories/Family for anti-ELL3 antibody

Antibodies; Enzymes; Polymerase

References

Lamesch, P., et al. Genomics 89(3):307-315(2007). Miller, T., et al. J. Biol. Chem. 275(41):32052-32056(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 13376768
• NCBI GeneID: 80237
• NCBI Accession #: NP_079441.1
• NCBI GenBank Nucleotide #: NM_025165.2
• UniProt Accession #: Q9HB65; Q6I9Z7; Q9H634; B3KQ66; B3KX08
• Molecular Weight: 45,361 Da
• NCBI Official Full Name: RNA polymerase II elongation factor ELL3
• NCBI Official Synonym Full Names: elongation factor RNA polymerase II-like 3
• NCBI Official Symbol: ELL3
• NCBI Protein Information: RNA polymerase II elongation factor ELL3
• UniProt Protein Name: RNA polymerase II elongation factor ELL3
• Protein Family: RNA polymerase II elongation factor
• UniProt Gene Name: ELL3
• UniProt Entry Name: ELL3_HUMAN

Uniprot Description

ELL3: Elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Belongs to the ELL/occludin family.

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: nucleoplasm; transcription elongation factor complex; nucleolus; nucleus

Biological Process: transcription from RNA polymerase II promoter; positive regulation of neurogenesis; RNA elongation; positive regulation of RNA elongation; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation