Rabbit anti-Human ELAVL2 Polyclonal Antibody | anti-ELAVL2 antibody

ELAVL2, ID (ELAVL2, HUB, ELAV-like protein 2, ELAV-like neuronal protein 1, Hu-antigen B, Nervous system-specific RNA-binding protein Hel-N1) (APC)

Cat. Num. AAA6295183

• Gene Names: ELAVL2; HUB; HELN1; HEL-N1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ELAVL2; Polyclonal Antibody; ID (ELAVL2; HUB; ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; Nervous system-specific RNA-binding protein Hel-N1) (APC); Nervous system-specific RNA-binding protein Hel-N1; anti-ELAVL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ELAVL2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ELAVL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-184 amino acids from the Central region of human ELAVL2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ELAVL2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ELAVL2 antibody detected the ELAVL2 protein (arrow).)

Immunohistochemistry (IHC)

(ELAVL2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ELAVL2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ELAVL2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ELAVL2 antibody

The protein encoded by this gene is a neural-specific RNA-binding protein that is known to bind to several 3' UTRs, including its own and also that of FOS and ID. The encoded protein may recognize a GAAA motif in the RNA. Three transcript variants encoding two different isoforms have been found for this gene.

Product Categories/Family for anti-ELAVL2 antibody

Antibodies; Binding Proteins

References

Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009). Lowe, J.K., et al. PLoS Genet. 5 (2), E1000365 (2009): D'Alessandro, V., et al. Cell. Oncol. 30(4):291-297(2008). Jonson, L., et al. Mol. Cell Proteomics 6(5):798-811(2007). Yano, M., et al. J. Biol. Chem. 280(13):12690-12699(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 283945529
• NCBI GeneID: 1993
• NCBI Accession #: NP_001164666.1; NP_001164668.1
• NCBI GenBank Nucleotide #: NM_001171195.1
• UniProt Accession #: Q12926; Q13235; Q59G15; Q8NEM4; Q9H1Q8; D3DRK3
• Molecular Weight: 39,504 Da
• NCBI Official Full Name: ELAV-like protein 2 isoform b
• NCBI Official Synonym Full Names: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• NCBI Official Symbol: ELAVL2
• NCBI Official Synonym Symbols: HUB; HELN1; HEL-N1
• NCBI Protein Information: ELAV-like protein 2; hu-antigen B; ELAV-like neuronal protein 1; nervous system-specific RNA-binding protein Hel-N1
• UniProt Protein Name: ELAV-like protein 2
• Protein Family: ELAV-like protein
• UniProt Gene Name: ELAVL2
• UniProt Synonym Gene Names: HUB; HuB
• UniProt Entry Name: ELAV2_HUMAN

NCBI Description

The protein encoded by this gene is a neural-specific RNA-binding protein that is known to bind to several 3' UTRs, including its own and also that of FOS and ID. The encoded protein may recognize a GAAA motif in the RNA. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

ELAVL2: Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR. Belongs to the RRM elav family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 9p21

Molecular Function: protein binding; mRNA 3'-UTR binding; nucleotide binding

Biological Process: regulation of transcription, DNA-dependent