Rabbit anti-Human EIF4H Polyclonal Antibody | anti-EIF4H antibody

EIF4H, ID (EIF4H, KIAA0038, WBSCR1, WSCR1, Eukaryotic translation initiation factor 4H, Williams-Beuren syndrome chromosomal region 1 protein) (AP)

Cat. Num. AAA6295127

• Gene Names: EIF4H; WSCR1; WBSCR1; eIF-4H
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF4H; Polyclonal Antibody; ID (EIF4H; KIAA0038; WBSCR1; WSCR1; Eukaryotic translation initiation factor 4H; Williams-Beuren syndrome chromosomal region 1 protein) (AP); Williams-Beuren syndrome chromosomal region 1 protein; anti-EIF4H antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-EIF4H antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EIF4H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-156 amino acids from the Central region of human EIF4H.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(EIF4H Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF4H Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(EIF4H Antibody (Center) western blot analysis in MDA-MB231,Hela cell line lysates (35ug/lane).This demonstrates the EIF4H antibody detected the EIF4H protein (arrow).)

Related Product Information for anti-EIF4H antibody

This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants.

Product Categories/Family for anti-EIF4H antibody

Antibodies; Translation Initiation Factors

References

Feng, T., et al. Hum. Genet. 128(3):269-280(2010). Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010). Mokas, S., et al. Mol. Biol. Cell 20(11):2673-2683(2009). Marintchev, A., et al. Cell 136(3):447-460(2009). Rozovsky, N., et al. RNA 14(10):2136-2148(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 14702180
• NCBI GeneID: 7458
• NCBI Accession #: NP_114381.1
• NCBI GenBank Nucleotide #: NM_031992.1
• UniProt Accession #: Q15056; A8K3R1; D3DXF6; D3DXF8
• Molecular Weight: 29.9 kDa
• NCBI Official Full Name: eukaryotic translation initiation factor 4H isoform 2
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4H
• NCBI Official Symbol: EIF4H
• NCBI Official Synonym Symbols: WSCR1; WBSCR1; eIF-4H
• NCBI Protein Information: eukaryotic translation initiation factor 4H
• UniProt Protein Name: Eukaryotic translation initiation factor 4H
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF4H
• UniProt Synonym Gene Names: KIAA0038; WBSCR1; WSCR1; eIF-4H
• UniProt Entry Name: IF4H_HUMAN

Uniprot Description

EIF4H: eukaryotic translation initiation factor 4H stimulates protein translation. May stimulate the activities of EIF4A, EIF4B and EIF4F during the steps involving mRNA binding and utilization in the initiation of protein biosynthesis. Binds mRNA. Two alternatively spliced isoforms have been described. The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.

Protein type: Translation; RNA-binding; Translation initiation

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: membrane; perinuclear region of cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; translation factor activity, nucleic acid binding; RNA binding; translation initiation factor activity; nucleotide binding

Biological Process: developmental growth; cellular protein metabolic process; translation; viral reproduction; sexual reproduction; translational initiation; gene expression; regulation of translational initiation

Disease: Williams-beuren Syndrome