Rabbit anti-Human EGF Polyclonal Antibody | anti-EGF antibody

EGF, CT (EGF, Pro-epidermal growth factor, Epidermal growth factor, Urogastrone) (Azide free) (HRP)

Cat. Num. AAA6294647

• Gene Names: EGF; URG; HOMG4
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EGF; Polyclonal Antibody; CT (EGF; Pro-epidermal growth factor; Epidermal growth factor; Urogastrone) (Azide free) (HRP); Urogastrone; anti-EGF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EGF antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: EGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 939-967 amino acids from the C-terminal region of human EGF.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EGF Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EGF antibody detected the EGF protein (arrow).)

Related Product Information for anti-EGF antibody

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-EGF antibody

Antibodies; Growth Factors; Cytokines

References

de Diesbach, M.T., et al. Exp. Cell Res. 316(19):3239-3253(2010). Xu, Z., et al. Biochem. Biophys. Res. Commun. 401(3):376-381(2010). Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010). Hommel, U., et al. J. Mol. Biol. 227(1):271-282(1992) Hernandez-Sotomayor, S.M., et al. J. Membr. Biol. 128(2):81-89(1992).

NCBI and Uniprot Product Information

• NCBI GI #: 166362728
• NCBI GeneID: 1950
• NCBI Accession #: NP_001954.2
• NCBI GenBank Nucleotide #: NM_001963.4
• UniProt Accession #: P01133; Q52LZ6; B4DRK7; E7EVD2; E9PBF0
• Molecular Weight: 129,746 Da
• NCBI Official Full Name: pro-epidermal growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor; beta-urogastrone
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]