Rabbit anti-Human E2F1 Polyclonal Antibody | anti-E2F1 antibody

E2F1, Phosphorylated (H357) (E2F1, RBBP3, Transcription factor E2F1, PBR3, PRB-binding protein E2F-1, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3) (Azide free) (HRP)

Cat. Num. AAA6294075

• Gene Names: E2F1; RBP3; E2F-1; RBAP1; RBBP3
• Reactivity: Human
• Applications: Western Blot, ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: E2F1; Polyclonal Antibody; Phosphorylated (H357) (E2F1; RBBP3; Transcription factor E2F1; PBR3; PRB-binding protein E2F-1; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3) (Azide free) (HRP); Retinoblastoma-binding protein 3; anti-E2F1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-E2F1 antibody: Western Blot (WB), ELISA (EIA), Dot Blot (DB)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-E2F1-H357 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding H357 of human E2F1.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-hE2F1-H357 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-E2F1 antibody

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Product Categories/Family for anti-E2F1 antibody

Antibodies; Retinoblastoma Proteins

References

Pulikkan, J.A., et al. Blood 115(9):1768-1778(2010). Paik, J.C., et al. J. Biol. Chem. 285(9):6348-6363(2010). Alla, V., et al. J. Natl. Cancer Inst. 102(2):127-133(2010). Zhou, C., et al. Mol. Endocrinol. 23(12):2000-2012(2009). Yang, X., et al. Genes Dev. 23(20):2388-2393(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 12669911
• NCBI GeneID: 1869
• NCBI Accession #: NP_005216.1
• NCBI GenBank Nucleotide #: NM_005225.2
• UniProt Accession #: Q01094; Q13143; Q92768
• Molecular Weight: 46,920 Da
• NCBI Official Full Name: transcription factor E2F1
• NCBI Official Synonym Full Names: E2F transcription factor 1
• NCBI Official Symbol: E2F1
• NCBI Official Synonym Symbols: RBP3; E2F-1; RBAP1; RBBP3
• NCBI Protein Information: transcription factor E2F1; PBR3; RBAP-1; RBBP-3; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1
• UniProt Protein Name: Transcription factor E2F1
• Protein Family: Transcription factor
• UniProt Gene Name: E2F1
• UniProt Synonym Gene Names: RBBP3; E2F-1; RBAP-1; RBBP-3
• UniProt Entry Name: E2F1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F1: a member of the E2F/DP family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Binds DNA cooperatively with Dp transcription factors in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.The Dp-1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It can mediate both cell proliferation and p53-dependent apoptosis.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity

Biological Process: Notch signaling pathway; transcription, DNA-dependent; apoptosis; positive regulation of transcription, DNA-dependent; mRNA stabilization; negative regulation of transcription from RNA polymerase II promoter; anoikis; positive regulation of fibroblast proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of transcription, DNA-dependent; DNA damage checkpoint; forebrain development; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle