Mouse CD86 Monoclonal Antibody | anti-CD86 antibody

MOUSE ANTI HUMAN CD86:RPE

Cat. Num. AAA213553

• Gene Names: CD86; B70; B7-2; B7.2; LAB72; CD28LG2
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD86; Monoclonal Antibody; MOUSE ANTI HUMAN CD86:RPE; anti-CD86 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BU63
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Sequence Length: 217

• Applicable Applications for anti-CD86 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
• Reconstitution: Reconstitute with 1 ml distilled water; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD86:FITC (MBS210618))

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD86:RPE)

Related Product Information for anti-CD86 antibody

Mouse anti Human CD86 antibody, clone Bu63 recognizes human CD86 also known as B7-2, a type I transmembrane protein expressed by monocytes and activated B cells (Engel et al. 1994). CD86 acts as a co-stimulaory molecule along with CD80 (Lanier et al. 1995) and is a ligand for CD28 and CTLA-4 (Azuma et al. 1993). CD86 is a member of the Immunoglobulin superfamily and carries an extracellular domain bearing both an Ig-v-like domain which contains the CTLA-4 binding site and an adjacent C2-like domain. CD86 plays an important role in co-stimulation of T cell proliferation (Freeman et al. 1993), IL-2 production (Ribot et al. 2012) and in the primary immune response (Schultze et al. 1996). Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997). Studies suggest that CD86 along with CD80 may be exploited as receptors for adenovirus entry into cells (Short et al. 2004 2006).

NCBI and Uniprot Product Information

• NCBI GI #: 332634950
• NCBI GeneID: 942
• NCBI Accession #: NP_001193853.1
• NCBI GenBank Nucleotide #: NM_001206924.1
• UniProt Accession #: P42081; Q13655; Q6FHB1; Q6GTS4; Q7M4L5; A0N0P0; B7Z2F3; B7Z702; E7ETN5; E9PC27
• Molecular Weight: 28,420 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD86 isoform 4
• NCBI Official Synonym Full Names: CD86 molecule
• NCBI Official Symbol: CD86
• NCBI Official Synonym Symbols: B70; B7-2; B7.2; LAB72; CD28LG2
• NCBI Protein Information: T-lymphocyte activation antigen CD86; B-lymphocyte activation antigen B7-2; BU63; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; FUN-1
• UniProt Protein Name: T-lymphocyte activation antigen CD86
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD86
• UniProt Synonym Gene Names: CD28LG2
• UniProt Entry Name: CD86_HUMAN

NCBI Description

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Uniprot Description

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: cell surface; intracellular membrane-bound organelle; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; receptor activity; receptor binding

Biological Process: response to drug; epidermal growth factor receptor signaling pathway; positive regulation of lymphotoxin A biosynthetic process; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; B cell activation; nerve growth factor receptor signaling pathway; negative regulation of T cell anergy; viral reproduction; T cell activation; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; myeloid dendritic cell differentiation; positive regulation of activated T cell proliferation; positive regulation of interleukin-4 biosynthetic process; positive regulation of T-helper 2 cell differentiation; cell-cell signaling; T cell proliferation during immune response; positive regulation of cell proliferation; T cell costimulation; toll-like receptor signaling pathway; innate immune response; immune response; response to yeast; defense response to virus; aging