Rabbit anti-Human DUX4 Polyclonal Antibody | anti-DUX4 antibody

DUX4, ID (DUX4, DUX10, Double homeobox protein 4, Double homeobox protein 10, Double homeobox protein 4/10) (Azide free) (HRP)

Cat. Num. AAA6293888

• Gene Names: DUX4L1; DUX4; DUX10
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DUX4; Polyclonal Antibody; ID (DUX4; DUX10; Double homeobox protein 4; Double homeobox protein 10; Double homeobox protein 4/10) (Azide free) (HRP); Double homeobox protein 4/10; anti-DUX4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DUX4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DUX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-283 amino acids from the Central region of human DUX4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DUX4 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DUX4 antibody detected the DUX4 protein (arrow).)

Related Product Information for anti-DUX4 antibody

DUX4 may be involved in transcriptional regulation.

Product Categories/Family for anti-DUX4 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

NCBI and Uniprot Product Information

• NCBI GI #: 194272172
• NCBI GeneID: 22947
• NCBI Accession #: NP_149418.3
• NCBI GenBank Nucleotide #: NM_033178.2
• UniProt Accession #: Q9UBX2
• Molecular Weight: 44,940 Da
• NCBI Official Full Name: double homeobox protein 4
• NCBI Official Synonym Full Names: double homeobox 4 like 1
• NCBI Official Symbol: DUX4L1
• NCBI Official Synonym Symbols: DUX4; DUX10
• UniProt Protein Name: Double homeobox protein 4
• Protein Family: Double homeobox protein
• UniProt Gene Name: DUX4
• UniProt Entry Name: DUX4_HUMAN

NCBI Description

This gene is located within a D4Z4 repeat array in the subtelomeric region of chromosome 4q. The D4Z4 repeat is polymorphic in length and a similar D4Z4 repeat array has been identified on chromosome 10. Each D4Z4 repeat unit has an open reading frame (named DUX4) that encodes two homeoboxes; the repeat-array and ORF is conserved in other mammals. There is no evidence for transcription of the gene at this locus though RT-PCR and in vitro expression experiments indicate that a telomeric paralog of this gene is transcribed in some haplotypes. Contraction of the macrosatellite repeat causes autosomal dominant facioscapulohumeral muscular dystrophy (FSHD). [provided by RefSeq, Jun 2014]

Uniprot Description

DUX4: May be involved in transcriptional regulation. Defects in DUX4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Severity is highly variable. Weakness is slowly progressive and about 20% of affected individuals eventually require a wheelchair. Approximately 70-90% of individuals have inherited the disease-causing deletion from a parent, and approximately 10-30% of affected individuals have FSHD as the result of a de novo deletion. Offsprings of an affected individual have a 50% chance of inheriting the deletion. Belongs to the paired homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 4q35