Rabbit anti-Human, Mouse Protein Kinase C, mu, Protein Kinase D Polyclonal Antibody | anti-Nell2 antibody

Protein Kinase C, mu, Protein Kinase D, phosphorylated (Ser742) (PKCm, PKD)

Cat. Num. AAA629307

• Gene Names: Nell2; mel91; R75516; A330108N19Rik
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Protein Kinase C; mu; Protein Kinase D; Polyclonal Antibody; phosphorylated (Ser742) (PKCm; PKD); Anti -Protein Kinase C; anti-Nell2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes human and mouse PKCm/PKD when phosphorylated at Ser742. Does not cross react with PKCa [pT497], b I&II [pT500], g [pT514], d [pT507], e [pT566], i [pT403], h [pT512], th [pT538], or z [pT410] as determined by peptide competition experiments.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

• Applicable Applications for anti-Nell2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide derived from a region of human PKCm/PKD that contains serine 742. Species sequence homology: mouse.
• Positive Control: K562 cells treated with PMA, or NIH3T3 cells treated with PDGF
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Nell2 antibody

Protein Kinase Cm (PKCm), is a ~140kD member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKCm (also known as Protein Kinase D or PKD) is implicated in the regulation of multiple cellular processes including Golgi organization and membrane transport in epithelial cells. PKCm is phosphorylated on serine 742 (serine 748 for the mouse sequence) in the activation loop in a PKC-dependent pathway, mainly by PKCh and PKCe. his is critical for its catalytic activity, substrate phosphorylation and role in activating the ERK1 MAP Kinase signaling cascade.

Product Categories/Family for anti-Nell2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 133922561
• NCBI GeneID: 54003
• NCBI Accession #: NP_058023.2
• NCBI GenBank Nucleotide #: NM_016743.2
• UniProt Accession #: Q61220; Q80UM5
• Molecular Weight: 91,432 Da
• NCBI Official Full Name: protein kinase C-binding protein NELL2
• NCBI Official Synonym Full Names: NEL-like 2
• NCBI Official Symbol: Nell2
• NCBI Official Synonym Symbols: mel91; R75516; A330108N19Rik
• NCBI Protein Information: protein kinase C-binding protein NELL2; NEL-like protein 2; nel-like 2 homolog
• UniProt Protein Name: Protein kinase C-binding protein NELL2
• Protein Family: Protein kinase C-binding protein
• UniProt Gene Name: Nell2
• UniProt Synonym Gene Names: Mel91
• UniProt Entry Name: NELL2_MOUSE

Uniprot Description

NELL2: is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009]

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; membrane; cytoplasm; dendrite; extracellular region; perikaryon

Molecular Function: heparin binding; identical protein binding; protein kinase C binding; calcium ion binding

Biological Process: regulation of growth; positive regulation of hormone secretion; development of secondary female sexual characteristics