Rabbit anti-Human, Mouse DNAJC5 Polyclonal Antibody | anti-DNAJC5 antibody

DNAJC5, ID (DNAJC5, CSP, DnaJ homolog subfamily C member 5, Cysteine string protein) (FITC)

Cat. Num. AAA6292853

• Gene Names: DNAJC5; CSP; NCL; CLN4; CLN4B; DNAJC5A
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DNAJC5; Polyclonal Antibody; ID (DNAJC5; CSP; DnaJ homolog subfamily C member 5; Cysteine string protein) (FITC); Cysteine string protein; anti-DNAJC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DNAJC5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DNAJC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 amino acids from the Central region of human DNAJC5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DNAJC5 Antibody (Center) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the DNAJC5 antibody detected the DNAJC5 protein (arrow).)

Western Blot (WB)

(DNAJC5 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DNAJC5 antibody detected the DNAJC5 protein (arrow).)

Related Product Information for anti-DNAJC5 antibody

This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. The encoded protein plays a role in membrane trafficking and protein folding, and has been shown to have anti-neurodegenerative properties. The encoded protein is known to play a role in cystic fibrosis and Huntington's disease. A pseudogene of this gene is located on the short arm of chromosome 8.

Product Categories/Family for anti-DNAJC5 antibody

Antibodies; Amino Acids

References

Johnson, J.N., et al. Biochem. Cell Biol. 88(2):157-165(2010). Schmidt, B.Z., et al. J. Biol. Chem. 284(7):4168-4178(2009). Greaves, J., et al. J. Biol. Chem. 283(36):25014-25026(2008). Park, J., et al. Am. J. Respir. Cell Mol. Biol. 39(1):68-76(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 45504382
• NCBI GeneID: 80331
• NCBI Accession #: NP_079495.1
• NCBI GenBank Nucleotide #: NM_025219.2
• UniProt Accession #: Q9H3Z4; Q9H3Z5; Q9H7H2; A8K0M0; B3KY68; E1P5G8
• Molecular Weight: 22,149 Da
• NCBI Official Full Name: dnaJ homolog subfamily C member 5
• NCBI Official Synonym Full Names: DnaJ (Hsp40) homolog, subfamily C, member 5
• NCBI Official Symbol: DNAJC5
• NCBI Official Synonym Symbols: CSP; NCL; CLN4; CLN4B; DNAJC5A
• NCBI Protein Information: dnaJ homolog subfamily C member 5; cysteine string protein alpha
• UniProt Protein Name: DnaJ homolog subfamily C member 5
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: DNAJC5
• UniProt Synonym Gene Names: CSP; CSP
• UniProt Entry Name: DNJC5_HUMAN

NCBI Description

This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. The encoded protein plays a role in membrane trafficking and protein folding, and has been shown to have anti-neurodegenerative properties. The encoded protein is known to play a role in cystic fibrosis and Huntington's disease. A pseudogene of this gene is located on the short arm of chromosome 8. [provided by RefSeq, Nov 2010]

Uniprot Description

DNAJC5: a cysteine string protein that inhibits N-type calcium channels which is blocked by huntingtin mutants. May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings. Modulates the maturation of cystic fibrosis transmembrane conductance regulator. Two splice-variant isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: synaptic vesicle; membrane; mitochondrion; lysosomal membrane; plasma membrane; melanosome

Molecular Function: ATP-dependent protein binding; glycoprotein binding

Biological Process: regulated secretory pathway; synaptic transmission; synaptic vesicle exocytosis; exocytosis; neurotransmitter secretion; negative regulation of neuron apoptosis