Rabbit anti-Human DDX6 Polyclonal Antibody | anti-DDX6 antibody

DDX6, ID (DDX6, HLR2, RCK, Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK) (FITC)

Cat. Num. AAA6291544

• Gene Names: DDX6; P54; RCK; HLR2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX6; Polyclonal Antibody; ID (DDX6; HLR2; RCK; Probable ATP-dependent RNA helicase DDX6; ATP-dependent RNA helicase p54; DEAD box protein 6; Oncogene RCK) (FITC); Oncogene RCK; anti-DDX6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DDX6 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DDX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354~384 amino acids from the Central region of human DDX6.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DDX6 Antibody (Center) western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).This demonstrates the DDX6 antibody detected the DDX6 protein (arrow).)

Immunohistochemistry (IHC)

(DDX6 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDX6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DDX6 antibody

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.

Product Categories/Family for anti-DDX6 antibody

Antibodies; DEAD Box Proteins

References

Broytman, O., et al. Neurobiol. Aging 30(12):1962-1974(2009). Han, J.W., et al. Nat. Genet. 41(11):1234-1237(2009). Minshall, N., et al. Mol. Biol. Cell 20(9):2464-2472(2009). Tritschler, F., et al. Mol. Cell 33(5):661-668(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 116241327
• NCBI GeneID: 1656
• UniProt Accession #: P26196; Q5D048
• Molecular Weight: 483
• NCBI Official Full Name: Probable ATP-dependent RNA helicase DDX6
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box helicase 6
• NCBI Official Symbol: DDX6
• NCBI Official Synonym Symbols: P54; RCK; HLR2
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX6; DEAD box-6; oncogene RCK; DEAD box protein 6; ATP-dependent RNA helicase p54; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX6
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DDX6
• UniProt Synonym Gene Names: HLR2; RCK
• UniProt Entry Name: DDX6_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012]

Uniprot Description

DDX6: an oncogenic ATP-dependent helicase of the DEAD box family. Interacts with argonaute proteins, Ago1 and Ago2, and is a general repressor of translation in human cells by miRNA-induced gene silencing. In the process of mRNA degradation, may play a role in mRNA decapping.

Protein type: RNA processing; Oncoprotein; EC 3.6.4.13; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: intracellular membrane-bound organelle; membrane; stress granule; cytoplasm; cytosol

Molecular Function: protein domain specific binding; protein binding; helicase activity; ATP binding; RNA helicase activity

Biological Process: gene expression; cytoplasmic mRNA processing body assembly; mRNA catabolic process, deadenylation-dependent decay