Mouse Chromodomain Helicase DNA Binding Protein 4 (CHD4) ELISA Kit | CHD4 elisa kit

Mouse Chromodomain Helicase DNA Binding Protein 4 (CHD4) ELISA Kit

Cat. Num. AAA450545

• Gene Names: CHD4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA
• Reactivity: Mouse
• Synonyms: Chromodomain Helicase DNA Binding Protein 4 (CHD4); Mouse Chromodomain Helicase DNA Binding Protein 4 (CHD4) ELISA Kit; Mi-2b; Mi2-BETA; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; CHD4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHD4. No significant cross-reactivity or interference between CHD4 and analogues was observed.
• Sequence Length: 1912

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CHD4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CHD4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHD4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CHD4 in mouse tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CHD4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CHD4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CHD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CHD4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 51599156
• NCBI GeneID: 1108
• NCBI Accession #: NP_001264.2
• NCBI GenBank Nucleotide #: NM_001273.3
• UniProt Accession #: Q14839; Q8IXZ5
• Molecular Weight: 220,848 Da
• NCBI Official Full Name: chromodomain-helicase-DNA-binding protein 4 isoform 1
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 4
• NCBI Official Symbol: CHD4
• NCBI Official Synonym Symbols: CHD-4; Mi-2b; SIHIWES; Mi2-BETA
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 4
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 4
• Protein Family: Chromodomain-helicase-DNA-binding protein
• UniProt Gene Name: CHD4
• UniProt Synonym Gene Names: CHD-4

NCBI Description

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

Uniprot Description

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.