Rabbit anti-Human, Mouse DDIT3 Polyclonal Antibody | anti-DDIT3 antibody

DDIT3, CT (A135) (DDIT3, CHOP, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, C/EBP-homologous protein, C/EBP-homologous protein 10, Growth arrest and DNA damage-inducible protein GADD153) (Biotin)

Cat. Num. AAA6291268

• Gene Names: DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; C/EBPzeta
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: DDIT3; Polyclonal Antibody; CT (A135) (DDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; C/EBP-homologous protein; C/EBP-homologous protein 10; Growth arrest and DNA damage-inducible protein GADD153) (Biotin); Growth arrest and DNA damage-inducible protein GADD153; anti-DDIT3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDIT3. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DDIT3 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 120-149 in the C-terminal region of human DDIT3. Species sequence homology: bovine, hamster
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in human skeletal muscle lysate (20ug/lane) using MBS6010216 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 19kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot analysis in PC-3 whole cell lysate (20ug/lane) using MBS6010216 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 19kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot analysis in PC-3 whole cell lysate (20ug/lane) using MBS6010216 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 19kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunofluorescence (IF)

(Fluorescent image of Hela cells stained withMBS6010216. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBS6010216 (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). DDIT3 immunoreactivity is localized to Cytoplasm significantly.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS6010216 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DDIT3 antibody

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Product Categories/Family for anti-DDIT3 antibody

Antibodies; Transcription Factors

References

Park, S.H., et al. J. Immunol. 185(9):5522-5530(2010). Goodall, J.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(41):17698-17703(2010). Zhang, H.M., et al. J. Virol. 84(17):8446-8459(2010). Cazanave, S.C., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (1), G236-G243 (2010): Wang, Y.L., et al. J. Exp. Clin. Cancer Res. 29, 54 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 21361118
• NCBI GeneID: 1649
• NCBI Accession #: NP_004074
• NCBI GenBank Nucleotide #: NM_004083.5
• UniProt Accession #: P35638; F8VS99
• Molecular Weight: 21.3kDa (189aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein isoform 2
• NCBI Official Synonym Full Names: DNA damage inducible transcript 3
• NCBI Official Symbol: DDIT3
• NCBI Official Synonym Symbols: CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; C/EBPzeta
• NCBI Protein Information: DNA damage-inducible transcript 3 protein
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• Protein Family: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: DDIT3
• UniProt Synonym Gene Names: CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10
• UniProt Entry Name: DDIT3_HUMAN

NCBI Description

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: Autophagy; Transcription factor; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: nucleoplasm; late endosome; nucleus; cytosol

Molecular Function: leucine zipper domain binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity; transcription corepressor activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; regulation of transcription in response to stress; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; unfolded protein response; negative regulation of transcription factor activity; blood vessel maturation; mRNA transcription from RNA polymerase II promoter; response to unfolded protein; response to starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; negative regulation of myoblast differentiation; response to DNA damage stimulus

Disease: Myxoid Liposarcoma