Rabbit anti-Human DCT Polyclonal Antibody | anti-DCT antibody

DCT, NT (DCT, TYRP2, L-dopachrome tautomerase, L-dopachrome Delta-isomerase, Tyrosinase-related protein 2) (AP)

Cat. Num. AAA6291189

• Gene Names: DCT; TRP-2; TYRP2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DCT; Polyclonal Antibody; NT (DCT; TYRP2; L-dopachrome tautomerase; L-dopachrome Delta-isomerase; Tyrosinase-related protein 2) (AP); Tyrosinase-related protein 2; anti-DCT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DCT.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-DCT antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 62-89 in the N-terminal region of human DCT, UniProt Accession #P40126.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(IHC analysis of MBS644568 in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Western Blot (WB)

(Western Blot analysis of MBS644568 in A375 cell line lysates (35ug/lane). DCT (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-DCT antibody

Antibodies; Disease Markers

References

1. Edwards, M., et al. PLoS Genet. 6 (3), E1000867 (2010). 2. Khong, H.T., et al. J. Immunol. 168(2):951-956(2002). 3. Sugimoto, H., et al. Biochemistry 38(11):3268-3279(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 193788640
• NCBI GeneID: 1638
• NCBI Accession #: NP_001123361.1
• NCBI GenBank Nucleotide #: NM_001129889.1
• UniProt Accession #: P40126; Q09GT4
• Molecular Weight: 59,145 Da
• NCBI Official Full Name: L-dopachrome tautomerase isoform 2
• NCBI Official Synonym Full Names: dopachrome tautomerase
• NCBI Official Symbol: DCT
• NCBI Official Synonym Symbols: TRP-2; TYRP2
• NCBI Protein Information: L-dopachrome tautomerase; DT; TRP2; dopachrome delta-isomerase; tyrosine-related protein 2; L-dopachrome Delta-isomerase; tyrosinase-related protein 2; dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
• UniProt Protein Name: L-dopachrome tautomerase
• Protein Family: dCTP deaminase
• UniProt Gene Name: DCT
• UniProt Synonym Gene Names: TYRP2; DCT; DT; TRP-2; TRP2
• UniProt Entry Name: TYRP2_HUMAN

Uniprot Description

DCT: Involved in regulating eumelanin and phaeomelanin levels. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; EC 5.3.3.12; Membrane protein, integral; Amino Acid Metabolism - tyrosine; Cell development/differentiation

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: melanosome membrane; integral to membrane; melanosome; cytosol

Molecular Function: copper ion binding; oxidoreductase activity; dopachrome isomerase activity

Biological Process: neuroblast division in the ventricular zone; positive regulation of neuroblast proliferation; epidermis development; pigmentation during development; melanin biosynthetic process from tyrosine; cell development