Goat Cholinergic Receptor, Nicotinic, alpha 7, Exons 5-10 Polyclonal Antibody | anti-CHRNA7 antibody

Cholinergic Receptor, Nicotinic, alpha 7, Exons 5-10 (CHRFAM7A, CHRNA7 and FAM7A Family with Sequence Similarity 7A, Exons A-E Fusion, CHRNA7, CHRNA7-DR1, CHRNA7-FAM7A Fusion Protein, D-10, MGC120482, MGC120483)

Cat. Num. AAA629073

• Gene Names: CHRNA7; NACHRA7; CHRNA7-2
• Reactivity: Human (Expected from Sequence Similarity)
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Cholinergic Receptor; Nicotinic; alpha 7; Exons 5-10; Polyclonal Antibody; Exons 5-10 (CHRFAM7A; CHRNA7 and FAM7A Family with Sequence Similarity 7A; Exons A-E Fusion; CHRNA7; CHRNA7-DR1; CHRNA7-FAM7A Fusion Protein; D-10; MGC120482; MGC120483); CHRFAM7A; CHRNA7-FAM7A; CHRNA7 (cholinergic receptor; nicotinic; exons 5-10) and FAM7A (family with sequence similarity 7A; exons A-E) fusion; MGC120483; alpha polypeptide 7; CHRNA7-FAM7A fusion; alpha 7 neuronal nicotinic acetylcholine receptor-FAM7A hybrid; alpha-7 nicotinic cholinergic receptor subunit; anti-CHRNA7 antibody

• Host: Goat
• Reactivity: Human (Expected from Sequence Similarity)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHRFAM7A.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence: Peptide with sequence QKYCIYQHFQFQ, from the N Terminus of the protein sequence according to NP_647536.1.

• Applicable Applications for anti-CHRNA7 antibody: ELISA (EL/EIA), Western Blot
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:1000.; Western blot: Preliminary experiments in Human Brain (Amygdala, Hippocampus, Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
• Immunogen: Synthetic peptide, QKYCIYQHFQFQ, from the N-Terminus of human CHRFAM7A.
• Positive Control: Human Brain (Amygdala, Hippocampus, Cerebellum) lysates
• Note: This antibody is expected to recognize reported isoform NP_647536.1 only.
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Related Product Information for anti-CHRNA7 antibody

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The family member CHRNA7, which is located on chromosome 15 in a region associated with several neuropsychiatric disorders, is partially duplicated and forms a hybrid with a novel gene from the family with sequence similarity 7 (FAM7A). Alternative splicing has been observed, and two variants exist, for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel. Although current evidence supports transcription of this hybrid gene, translation of the nicotinic acetylcholine receptor-like protein-encoding open reading frames has not been confirmed.

Product Categories/Family for anti-CHRNA7 antibody

Antibodies; Abs to Receptors; Cholinergic

NCBI and Uniprot Product Information

• NCBI GI #: 457737
• NCBI GeneID: 1139
• UniProt Accession #: P36544; Q15826; Q96RH2; Q99555; Q9BXH0; A8K7Q4; Q5W554
• NCBI Official Full Name: cholinergic receptor, nicotinic, alpha polypeptide 7
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha 7
• NCBI Official Symbol: CHRNA7
• NCBI Official Synonym Symbols: NACHRA7; CHRNA7-2
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-7; OTTHUMP00000159615; a7 nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; alpha 7 neuronal nicotinic acetylcholine receptor; cholinergic receptor, nicotinic, alpha polypeptide 7; neuronal acetylcholine receptor protein, alpha-7 chain
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-7
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA7
• UniProt Synonym Gene Names: NACHRA7
• UniProt Entry Name: ACHA7_HUMAN

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

nAChRA7: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 7/CHRNA7 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, cation; Membrane protein, multi-pass; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; plasma membrane; integral to membrane; cell junction

Molecular Function: toxin binding; protein homodimerization activity; chloride channel regulator activity; beta-amyloid binding; acetylcholine-gated cation channel activity; acetylcholine receptor activity; nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding

Biological Process: cellular calcium ion homeostasis; response to nicotine; synaptic transmission; positive regulation of angiogenesis; activation of MAPK activity; calcium ion transport; positive regulation of cell proliferation; response to hypoxia; ion transport; negative regulation of tumor necrosis factor production; cognition; signal transduction; memory

Disease: Chromosome 15q13.3 Deletion Syndrome