Rabbit anti-Human CYP2D6 Polyclonal Antibody | anti-CYP2D6 antibody

CYP2D6, NT (CYP2D6, CYP2DL1, Cytochrome P450 2D6, CYPIID6, Cytochrome P450-DB1, Debrisoquine 4-hydroxylase) (APC)

Cat. Num. AAA6290508

• Gene Names: CYP2D6; CPD6; CYP2D; CYP2DL1; CYPIID6; P450C2D; P450DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; P450-DB1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CYP2D6; Polyclonal Antibody; NT (CYP2D6; CYP2DL1; Cytochrome P450 2D6; CYPIID6; Cytochrome P450-DB1; Debrisoquine 4-hydroxylase) (APC); Debrisoquine 4-hydroxylase; anti-CYP2D6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CYP2D6 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CYP2D6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-105 amino acids from the N-terminal region of human CYP2D6.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CYP2D6 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the CYP2D6 antibody detected the CYP2D6 protein (arrow).)

Immunohistochemistry (IHC)

(CYP2D6 Antibody (N-term) (AP14520a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP2D6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CYP2D6 antibody

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 20% of commonly prescribed drugs. Its substrates include debrisoquine, an adrenergic-blocking drug; sparteine and propafenone, both anti-arrythmic drugs; and amitryptiline, an anti-depressant. The gene is highly polymorphic in the population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. The gene is located near two cytochrome P450 pseudogenes on chromosome 22q13.1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-CYP2D6 antibody

Antibodies; Enzymes; Cytochrome

References

Abduljalil, K., et al. Clin. Pharmacol. Ther. 88(5):643-651(2010). Novalbos, J., et al. J Clin Psychopharmacol 30(5):504-511(2010). Thompson, A.M., et al. Breast Cancer Res. Treat. (2010). In press: Gonzalez-Tejera, G., et al. P R Health Sci J 29(3):299-304(2010). Abraham, J.E., et al. Breast Cancer Res. 12 (4), R64 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 392513721
• NCBI GeneID: 1565
• NCBI Accession #: NP_000097.3
• NCBI GenBank Nucleotide #: NM_000106.5
• UniProt Accession #: P10635; Q16752; Q2XND6; Q2XND7; Q2XNE0; Q6B012; Q6NXU8
• Molecular Weight: 55,769 Da
• NCBI Official Full Name: cytochrome P450 2D6 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily D, polypeptide 6
• NCBI Official Symbol: CYP2D6
• NCBI Official Synonym Symbols: CPD6; CYP2D; CYP2DL1; CYPIID6; P450C2D; P450DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; P450-DB1
• NCBI Protein Information: cytochrome P450 2D6; cytochrome P450-DB1; microsomal monooxygenase; xenobiotic monooxygenase; debrisoquine 4-hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, family 2, subfamily D, polypeptide 7 pseudogene 2; cytochrome P450, family 2, sub
• UniProt Protein Name: Cytochrome P450 2D6
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2D6
• UniProt Synonym Gene Names: CYP2DL1
• UniProt Entry Name: CP2D6_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 20% of commonly prescribed drugs. Its substrates include debrisoquine, an adrenergic-blocking drug; sparteine and propafenone, both anti-arrythmic drugs; and amitryptiline, an anti-depressant. The gene is highly polymorphic in the population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. The gene is located near two cytochrome P450 pseudogenes on chromosome 22q13.1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2D6: Responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It is involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.14.1; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum

Molecular Function: iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxidoreductase activity; drug binding; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; coumarin metabolic process; monoterpenoid metabolic process; isoquinoline alkaloid metabolic process; negative regulation of binding; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process; alkaloid metabolic process; alkaloid catabolic process; heterocycle metabolic process; drug metabolic process

Disease: Drug Metabolism, Poor, Cyp2d6-related