Rabbit c-Jun Polyclonal Antibody | anti-C-JUN antibody

c-Jun, phosphorylated (Tyr170) (Transcription Factor AP-1, Activator Protein 1, AP1, Proto-oncogene c-Jun, V-jun Avian Sarcoma Virus 17 Oncogene Homolog, p39, JUN)

Cat. Num. AAA629001

• Gene Names: C-JUN; Jun
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: c-Jun; Polyclonal Antibody; phosphorylated (Tyr170) (Transcription Factor AP-1; Activator Protein 1; AP1; Proto-oncogene c-Jun; V-jun Avian Sarcoma Virus 17 Oncogene Homolog; p39; JUN); Anti -c-Jun; anti-C-JUN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human c-Jun when phosphorylated at tyrosine 170. Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-C-JUN antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500-1:1,000
• Immunogen: Synthetic phosphopeptide corresponding to human c-Jun around the phosphorylation site of tyrosine 170 (P-V-YP-A-N).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of c-Jun (Tyr170) in HeLa cells.)

Related Product Information for anti-C-JUN antibody

The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Product Categories/Family for anti-C-JUN antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1911701
• NCBI GeneID: 396913
• UniProt Accession #: P56432
• Molecular Weight: 35,629 Da
• NCBI Official Full Name: c-Jun
• NCBI Official Symbol: C-JUN
• NCBI Official Synonym Symbols: Jun
• NCBI Protein Information: transcription factor AP-1; AP1; activator protein 1; proto-oncogene c-jun; V-jun avian sarcoma virus 17 oncogene homolog
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: JUN
• UniProt Entry Name: JUN_PIG

Uniprot Description

Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Subunit structure: Heterodimer with either FOS or BATF3 or ATF7

By similarity. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with MYBBP1A, SP1, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with HIVEP3. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta. Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex

By similarity. Interacts with RNF187

Subcellular location: Nucleus

Post-translational modification: Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC

Sequence similarities: Belongs to the bZIP family. Jun subfamily.Contains 1 bZIP domain.