Rabbit anti-Human CPSF7 Polyclonal Antibody | anti-CPSF7 antibody

CPSF7, CT (CPSF7, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59kD subunit, Pre-mRNA cleavage factor Im 59kD subunit) (FITC)

Cat. Num. AAA6288464

• Gene Names: CPSF7; CFIm59
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPSF7; Polyclonal Antibody; CT (CPSF7; Cleavage and polyadenylation specificity factor subunit 7; Cleavage and polyadenylation specificity factor 59kD subunit; Pre-mRNA cleavage factor Im 59kD subunit) (FITC); Pre-mRNA cleavage factor Im 59kD subunit; anti-CPSF7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CPSF7 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CPSF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 434-463 amino acids from the C-terminal region of human CPSF7.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CPSF7 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPSF7 antibody detected the CPSF7 protein (arrow).)

Flow Cytometry (FC/FACS)

(CPSF7 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CPSF7 antibody

CPSF7 is probable a component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.

Product Categories/Family for anti-CPSF7 antibody

Antibodies; Proteins

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). Lim, J., et al. Cell 125(4):801-814(2006). Brill, L.M., et al. Anal. Chem. 76(10):2763-2772(2004). Zhou, Z., et al. Nature 419(6903):182-185(2002). Ruegsegger, U., et al. J. Biol. Chem. 271(11):6107-6113(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 209862881
• NCBI GeneID: 79869
• NCBI Accession #: NP_001129512.1; NP_001136037.1; NP_079087.3
• NCBI GenBank Nucleotide #: NM_001136040.2
• UniProt Accession #: Q8N684; Q7Z3H9; Q9H025; Q9H9V1; B3KU04; C9K0Q4
• Molecular Weight: 56,375 Da
• NCBI Official Full Name: cleavage and polyadenylation specificity factor subunit 7 isoform 2
• NCBI Official Synonym Full Names: cleavage and polyadenylation specific factor 7
• NCBI Official Symbol: CPSF7
• NCBI Official Synonym Symbols: CFIm59
• NCBI Protein Information: cleavage and polyadenylation specificity factor subunit 7
• UniProt Protein Name: Cleavage and polyadenylation specificity factor subunit 7
• Protein Family: Cleavage and polyadenylation specificity factor
• UniProt Gene Name: CPSF7
• UniProt Synonym Gene Names: CFIm59; CPSF 59 kDa subunit
• UniProt Entry Name: CPSF7_HUMAN

NCBI Description

Cleavage factor Im (CFIm) is one of six factors necessary for correct cleavage and polyadenylation of pre-mRNAs. CFIm is composed of three different subunits of 25, 59, and 68 kDa, and it functions as a heterotetramer, with a dimer of the 25 kDa subunit binding to two of the 59 or 68 kDa subunits. The protein encoded by this gene represents the 59 kDa subunit, which can interact with the splicing factor U2 snRNP Auxiliary Factor (U2AF) 65 to link the splicing and polyadenylation complexes. [provided by RefSeq, Oct 2016]

Uniprot Description

CPSF7: probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates. Probable component of the cleavage factor Im (CFIm) complex, composed at least of NUDT21/CPSF5 and CPSF6 or CPSF7. Two alternatively-spliced isoforms have been described.

Protein type: RNA splicing; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: nucleoplasm; membrane; nucleus; mRNA cleavage factor complex

Molecular Function: protein binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; protein tetramerization