Western Blot (WB)
(Western blot analysis of GLRX2 antibody (C-term T135) in mouse cerebellum tissue lysates (35ug/lane). GLRX2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse Glutaredoxin-2, Mitochondrial, T135 Polyclonal Antibody | anti-GLRX2 antibody
Glutaredoxin-2, Mitochondrial, CT, T135 (GLRX2, GRX2, CGI-133)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Western Blot (WB)
(Western blot analysis of GLRX2 antibody (C-term T135) in mouse cerebellum tissue lysates (35ug/lane). GLRX2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of GLRX2 antibody (C-term T135) in mouse cerebellum tissue lysates (35ug/lane). GLRX2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain reacted with GLRX2 Antibody (C-term T135), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain reacted with GLRX2 Antibody (C-term T135), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of hela cells using GLRX2 Antibody (C-term T135)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of hela cells using GLRX2 Antibody (C-term T135)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
Glutaredoxins (e.g., GLRX; MIM 600443) are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions.[supplied by OMIM]
Uniprot Description
GLRX2: Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release. Belongs to the glutaredoxin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Oxidoreductase
Chromosomal Location of Human Ortholog: 1q31.2
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; mitochondrion; nucleus
Molecular Function: 2 iron, 2 sulfur cluster binding; electron carrier activity; metal ion binding; protein disulfide isomerase activity; protein disulfide oxidoreductase activity; arsenate reductase (glutaredoxin) activity; glutathione disulfide oxidoreductase activity
Biological Process: response to organic substance; response to temperature stimulus; DNA protection; glutathione metabolic process; response to hydrogen peroxide; protein folding; regulation of transcription, DNA-dependent; cell redox homeostasis; apoptosis; regulation of signal transduction; response to redox state; cell differentiation
Research Articles on GLRX2
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Product Notes
The GLRX2 glrx2 (Catalog #AAA628816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glutaredoxin-2, Mitochondrial, CT, T135 (GLRX2, GRX2, CGI-133) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glutaredoxin-2, Mitochondrial, T135 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the GLRX2 glrx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutaredoxin-2, Mitochondrial, T135, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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