Rabbit anti-Human COX10 Polyclonal Antibody | anti-COX10 antibody

COX10, CT (COX10, Protoheme IX farnesyltransferase, mitochondrial, Heme O synthase) (PE)

Cat. Num. AAA6288064

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COX10; Polyclonal Antibody; CT (COX10; Protoheme IX farnesyltransferase; mitochondrial; Heme O synthase) (PE); Heme O synthase; anti-COX10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-COX10 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: COX10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-414 amino acids from the C-terminal region of human COX10.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(COX10 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the COX10 antibody detected the COX10 protein (arrow).)

Immunohistochemistry (IHC)

(COX10 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COX10 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(COX10 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-COX10 antibody

Antibodies; Mitochondrial Proteins

References

Chen, Z., et al. Oncogene 29(30):4362-4368(2010). Vitali, M., et al. J Neural Transm 116(12):1635-1641(2009). Dassa, E.P., et al. EMBO Mol Med 1(1):30-36(2009). Veluthakal, R., et al. Diabetes 56(1):204-210(2007). Coenen, M.J., et al. Ann. Neurol. 56(4):560-564(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 17921982
• NCBI GeneID: 1352
• NCBI Accession #: NP_001294.2
• NCBI GenBank Nucleotide #: NM_001303.3
• UniProt Accession #: Q12887; O15334; Q969F7; B2R6U5
• Molecular Weight: 48,910 Da
• NCBI Official Full Name: protoheme IX farnesyltransferase, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase assembly homolog 10 (yeast)
• NCBI Official Symbol: COX10
• NCBI Protein Information: protoheme IX farnesyltransferase, mitochondrial; heme O synthase; heme A: farnesyltransferase; cytochrome c oxidase subunit X; cytochrome c oxidase assembly protein; COX10 homolog, cytochrome c oxidase assembly protein, heme A: farnesyltransferase
• UniProt Protein Name: Protoheme IX farnesyltransferase, mitochondrial
• Protein Family: Probable protoheme IX farnesyltransferase
• UniProt Gene Name: COX10
• UniProt Entry Name: COX10_HUMAN