Rabbit anti-Human, Mouse CLDN22 Polyclonal Antibody | anti-CLDN22 antibody

CLDN22, ID (CLDN22, Claudin-22) (PE)

Cat. Num. AAA6286491

• Gene Names: CLDN22; CLDN21
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CLDN22; Polyclonal Antibody; ID (CLDN22; Claudin-22) (PE); Claudin-22; anti-CLDN22 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLDN22. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CLDN22 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 90-117 in the central region of human CLDN22.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane), usingMBS6002237. This demonstrates that MBS6002237 detected the CLDN22 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane),MBS6006209. This demonstrates that MBS6002237 detected the CLDN22 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells (right histogram), using MBS6002237 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CLDN22 antibody

Antibodies; Claudin

References

1. Lal-Nag, M., et al. Genome Biol. 10 (8), 235 (2009). 2. Krause, G., et al. Biochim. Biophys. Acta 1778(3):631-645(2008). 3. Hillier, L.W., et al. Nature 434(7034):724-731(2005). 4. Katoh, M., et al. Int. J. Mol. Med. 11(6):683-689(2003). 5. Gonzalez-Mariscal, L., et al. Prog. Biophys. Mol. Biol. 81(1):1-44(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 162417973
• NCBI GeneID: 53842
• NCBI Accession #: NP_001104789.1
• NCBI GenBank Nucleotide #: NM_001111319.1
• UniProt Accession #: Q8N7P3
• Molecular Weight: 24,509 Da
• NCBI Official Full Name: claudin-22
• NCBI Official Synonym Full Names: claudin 22
• NCBI Official Symbol: CLDN22
• NCBI Official Synonym Symbols: CLDN21
• NCBI Protein Information: claudin-22
• UniProt Protein Name: Claudin-22
• Protein Family: Claudin
• UniProt Gene Name: CLDN22

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is intronless and overlaps the 3' UTR of the WWC2 gene (GeneID: 80014) on the opposite strand. [provided by RefSeq, Aug 2010]

Uniprot Description

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.