Immunohistochemistry (IHC)
(CKAP4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CKAP4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human CKAP4 Polyclonal Antibody | anti-CKAP4 antibody
CKAP4, CT (CKAP4, Cytoskeleton-associated protein 4, 63-kD cytoskeleton-linking membrane protein) (APC)
Gene Names
CKAP4; p63; CLIMP-63; ERGIC-63
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CKAP4; Polyclonal Antibody; CT (CKAP4; Cytoskeleton-associated protein 4; 63-kD cytoskeleton-linking membrane protein) (APC); 63-kDa cytoskeleton-linking membrane protein; anti-CKAP4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-CKAP4 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CKAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 581-602 amino acids from the C-terminal region of human CKAP4.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunohistochemistry (IHC)
(CKAP4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CKAP4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CKAP4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CKAP4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Western Blot (WB)
(CKAP4 Antibody (C-term) western blot analysis in 293,NCI-H460 cell line lysates (35ug/lane).This demonstrates the CKAP4 antibody detected the CKAP4 protein (arrow).)
Western Blot (WB)
(CKAP4 Antibody (C-term) western blot analysis in 293,NCI-H460 cell line lysates (35ug/lane).This demonstrates the CKAP4 antibody detected the CKAP4 protein (arrow).)
Related Product Information for anti-CKAP4 antibody
CKAP4 is a substrate of DHHC2, a putative tumor suppressor. CK MNF116 and CKAP4 were useful in identifying squamous cell carcinomas with single cell infiltration. These results suggest a role for CKAP4 in tumor.
Product Categories/Family for anti-CKAP4 antibody
References
Kanner, W.A., et al. J. Cutan. Pathol. 37(7):744-750(2010). Tomkova, H., et al. Eur J Dermatol 20(3):411-413(2010). Lefkimmiatis, K., et al. Cancer Res. 69(22):8563-8571(2009). Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009). Bates, S.R., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (4), L658-L669 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,022 Da
NCBI Official Full Name
cytoskeleton-associated protein 4
NCBI Official Synonym Full Names
cytoskeleton associated protein 4
NCBI Official Symbol
CKAP4
NCBI Official Synonym Symbols
p63; CLIMP-63; ERGIC-63
NCBI Protein Information
cytoskeleton-associated protein 4
UniProt Protein Name
Cytoskeleton-associated protein 4
Protein Family
UniProt Gene Name
CKAP4
UniProt Synonym Gene Names
Climp-63; p63
UniProt Entry Name
CKAP4_HUMAN
Uniprot Description
CKAP4: High-affinity epithelial cell surface receptor for APF.
Protein type: Endoplasmic reticulum; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12q23.3
Cellular Component: endoplasmic reticulum membrane; cytoskeleton; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; integral to membrane; plasma membrane; lipid particle
Research Articles on CKAP4
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Product Notes
The CKAP4 ckap4 (Catalog #AAA6286262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CKAP4, CT (CKAP4, Cytoskeleton-associated protein 4, 63-kD cytoskeleton-linking membrane protein) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CKAP4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CKAP4 ckap4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CKAP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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