Rabbit anti-Human CHMP2B Polyclonal Antibody | anti-CHMP2B antibody

CHMP2B, NT (CHMP2B, Charged multivesicular body protein 2b, CHMP2.5, Chromatin-modifying protein 2b, Vacuolar protein sorting-associated protein 2-2) (FITC)

Cat. Num. AAA6285670

• Gene Names: CHMP2B; DMT1; ALS17; VPS2B; VPS2-2; CHMP2.5
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHMP2B; Polyclonal Antibody; NT (CHMP2B; Charged multivesicular body protein 2b; CHMP2.5; Chromatin-modifying protein 2b; Vacuolar protein sorting-associated protein 2-2) (FITC); Vacuolar protein sorting-associated protein 2-2; anti-CHMP2B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CHMP2B antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CHMP2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CHMP2B.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CHMP2B Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the CHMP2B antibody detected the CHMP2B protein (arrow).)

Immunohistochemistry (IHC)

(CHMP2B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CHMP2B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CHMP2B antibody

This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration.

Product Categories/Family for anti-CHMP2B antibody

Antibodies; Chromatin Proteins

References

Kaivorinne, A.L., et al. Eur. J. Neurol. 17(11):1393-1395(2010). Ghanim, M., et al. J. Neurol. (2010). In press: Yamazaki, Y., et al. Neurosci. Lett. 477(2):86-90(2010). Tsai, C.P., et al. Neurobiol. Aging (2010). In press: Cox, L.E., et al. PLoS ONE 5 (3), E9872 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 40254866
• NCBI GeneID: 25978
• NCBI Accession #: NP_054762.2
• NCBI GenBank Nucleotide #: NM_014043.3
• UniProt Accession #: Q9UQN3; Q53HC7; Q9Y4U6; B4DJG8
• Molecular Weight: 26.1 kDa (233aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: charged multivesicular body protein 2b isoform 1
• NCBI Official Synonym Full Names: charged multivesicular body protein 2B
• NCBI Official Symbol: CHMP2B
• NCBI Official Synonym Symbols: DMT1; ALS17; VPS2B; VPS2-2; CHMP2.5
• NCBI Protein Information: charged multivesicular body protein 2b
• UniProt Protein Name: Charged multivesicular body protein 2b
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP2B
• UniProt Synonym Gene Names: CHMP2b; Vps2-2; hVps2-2
• UniProt Entry Name: CHM2B_HUMAN

Uniprot Description

CHMP2B: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Defects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3). FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits. Defects in CHMP2B are the cause of amyotrophic lateral sclerosis type 17 (ALS17). An adult-onset progressive neurodegenerative disorder with predominantly lower motor neuron involvement, manifest as muscle weakness and wasting of the upper and lower limbs, bulbar signs, and respiratory insufficiency. Belongs to the SNF7 family.

Chromosomal Location of Human Ortholog: 3p11.2

Cellular Component: nucleoplasm; mitochondrion; late endosome membrane; lysosome; late endosome; cytoplasm; plasma membrane; intracellular; nucleus; cytosol; endosome

Molecular Function: protein domain specific binding; protein binding

Biological Process: protein transport; endosome organization and biogenesis; viral reproduction; cell separation during cytokinesis; vacuolar transport; viral infectious cycle; cognition; endosome transport; mitotic metaphase plate congression; nuclear organization and biogenesis

Disease: Frontotemporal Dementia, Chromosome 3-linked; Amyotrophic Lateral Sclerosis 17