Rabbit anti-Human, Mouse CERK Polyclonal Antibody | anti-CERK antibody

CERK, CT (CERK, KIAA1646, Ceramide kinase, Acylsphingosine kinase, Lipid kinase 4) (APC)

Cat. Num. AAA6285239

• Gene Names: CERK; LK4; hCERK; dA59H18.2; dA59H18.3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CERK; Polyclonal Antibody; CT (CERK; KIAA1646; Ceramide kinase; Acylsphingosine kinase; Lipid kinase 4) (APC); Lipid kinase 4; anti-CERK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CERK antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CERK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495~524 amino acids from the C-terminal region of human CERK.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-CERK Pab is used in Western blot to detect CERK in mouse heart tissue lysate (Lane 1) and A2058 cell lysate (Lane 2).)

Related Product Information for anti-CERK antibody

Ceramide kinases convert the sphingolipid metabolite ceramide into ceramide-1-phosphate, both key mediators of cellular apoptosis and survival. Ceramide metabolism plays an essential role in the viability of neuronal cells, the membranes of which are particularly rich in sphingolipids. CERK catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. This enzyme acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8 dihydroceramide), and to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. High level expression is noted in heart, brain, skeletal muscle, kidney and liver; moderate expression in peripheral blood leukocytes and thymus; and low expression in spleen, small intestine, placenta and lung.

Product Categories/Family for anti-CERK antibody

Antibodies; Protein Kinases

References

J. Biol. Chem. 279 (17), 17570-17577 (2004). J. Biol. Chem. 278 (40), 38206-38213 (2003). J. Biol. Chem. 277 (26), 23294-23300 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 20336726
• NCBI GeneID: 64781
• NCBI Accession #: NP_073603
• NCBI GenBank Nucleotide #: NM_022766
• UniProt Accession #: Q8TCT0; Q6NX59; Q9BYB3; Q9UGE5; A0JNT4; A8K611
• Molecular Weight: 37,781 Da
• NCBI Official Full Name: ceramide kinase
• NCBI Official Synonym Full Names: ceramide kinase
• NCBI Official Symbol: CERK
• NCBI Official Synonym Symbols: LK4; hCERK; dA59H18.2; dA59H18.3
• NCBI Protein Information: ceramide kinase; acylsphingosine kinase; lipid kinase 4; lipid kinase LK4
• UniProt Protein Name: Ceramide kinase
• Protein Family: Ceramide kinase
• UniProt Gene Name: CERK
• UniProt Synonym Gene Names: KIAA1646; hCERK; LK4

Uniprot Description

Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.