Rabbit anti-Human CELA3A Polyclonal Antibody | anti-CELA3A antibody

CELA3A, ID (CELA3A, ELA3, ELA3A, Chymotrypsin-like elastase family member 3A, Elastase IIIA, Elastase-3A, Protease E) (APC)

Cat. Num. AAA6285030

• Gene Names: CELA3A; ELA3; ELA3A
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CELA3A; Polyclonal Antibody; ID (CELA3A; ELA3; ELA3A; Chymotrypsin-like elastase family member 3A; Elastase IIIA; Elastase-3A; Protease E) (APC); Protease E; anti-CELA3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CELA3A antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CELA3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-186 amino acids from the Central region of human CELA3A.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CELA3A Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CELA3A antibody detected the CELA3A protein (arrow).)

Immunohistochemistry (IHC)

(CELA3A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CELA3A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CELA3A antibody

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1.

Product Categories/Family for anti-CELA3A antibody

Antibodies; Elastase

References

Shimada, S., et al. Int. J. Mol. Med. 10(5):599-603(2002). Shirasu, Y., et al. J. Biochem. 104(2):259-264(1988) Tani, T., et al. J. Biol. Chem. 263(3):1231-1239(1988).

NCBI and Uniprot Product Information

• NCBI GI #: 236460050
• NCBI GeneID: 10136
• NCBI Accession #: NP_005738.4
• NCBI GenBank Nucleotide #: NM_005747.4
• UniProt Accession #: P09093; Q9BRW4; B1AQ53
• Molecular Weight: 29,489 Da
• NCBI Official Full Name: chymotrypsin-like elastase family member 3A preproprotein
• NCBI Official Synonym Full Names: chymotrypsin like elastase family member 3A
• NCBI Official Symbol: CELA3A
• NCBI Official Synonym Symbols: ELA3; ELA3A
• NCBI Protein Information: chymotrypsin-like elastase family member 3A
• UniProt Protein Name: Chymotrypsin-like elastase family member 3A
• Protein Family: Chymotrypsin-like elastase family
• UniProt Gene Name: CELA3A
• UniProt Synonym Gene Names: ELA3; ELA3A
• UniProt Entry Name: CEL3A_HUMAN

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq, Jul 2008]

Uniprot Description

CELA3A: Efficient protease with alanine specificity but only little elastolytic activity. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: EC 3.4.21.70; Protease

Chromosomal Location of Human Ortholog: 1p36.12

Molecular Function: serine-type endopeptidase activity

Biological Process: cholesterol metabolic process; digestion; proteolysis