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Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Rabbit anti-Human, Mouse CDKL1 Polyclonal Antibody | anti-CDKL1 antibody

CDKL1, CT (CDKL1, Cyclin-dependent kinase-like 1, Protein kinase p42 KKIALRE, Serine/threonine-protein kinase KKIALRE) (PE)

Gene Names
CDKL1; P42; KKIALRE
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
CDKL1; Polyclonal Antibody; CT (CDKL1; Cyclin-dependent kinase-like 1; Protein kinase p42 KKIALRE; Serine/threonine-protein kinase KKIALRE) (PE); Serine/threonine-protein kinase KKIALRE; anti-CDKL1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-CDKL1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CDKL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293~323 amino acids from the C-terminal region of human CDKL1.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(The anti-CDKL1 Pab is used in Western blot to detect CDKL1 in mouse kidney tissue lysate.)

Western Blot (WB) (The anti-CDKL1 Pab is used in Western blot to detect CDKL1 in mouse kidney tissue lysate.)

Western Blot (WB)

(Western blot analysis of CDKL1 (arrow) using rabbit polyclonal CDKL1 Antibody (H300). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDKL1 gene.)

Western Blot (WB) (Western blot analysis of CDKL1 (arrow) using rabbit polyclonal CDKL1 Antibody (H300). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDKL1 gene.)

Western Blot (WB)

(CDKL1 Antibody (H300) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CDKL1 antibody detected the CDKL1 protein (arrow).)

Western Blot (WB) (CDKL1 Antibody (H300) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CDKL1 antibody detected the CDKL1 protein (arrow).)
Related Product Information for anti-CDKL1 antibody
This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the nucleus. Alternative splice variants encoding different protein isoforms have been described, but their full-length nature has not been determined.
Product Categories/Family for anti-CDKL1 antibody
References
Meyerson, M., et al., EMBO J. 11(8):2909-2917 (1992).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,671 Da
NCBI Official Full Name
cyclin-dependent kinase-like 1
NCBI Official Synonym Full Names
cyclin-dependent kinase-like 1 (CDC2-related kinase)
NCBI Official Symbol
CDKL1
NCBI Official Synonym Symbols
P42; KKIALRE
NCBI Protein Information
cyclin-dependent kinase-like 1; CDC2-related kinase 1; protein kinase p42 KKIALRE; serine/threonine protein kinase KKIALRE; serine/threonine-protein kinase KKIALRE
UniProt Protein Name
Cyclin-dependent kinase-like 1
Protein Family
UniProt Gene Name
CDKL1
UniProt Entry Name
CDKL1_HUMAN

NCBI Description

This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the nucleus. Alternative splice variants encoding different protein isoforms have been described, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CDKL1: a protein kinase of the CDKL family. Accumulates primarily in the nucleus.

Protein type: Kinase, protein; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Cell cycle regulation; CMGC group; CDKL family

Chromosomal Location of Human Ortholog: 14q21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase activity; ATP binding

Biological Process: regulation of cell cycle; heart development; protein amino acid phosphorylation

Research Articles on CDKL1

Similar Products

Product Notes

The CDKL1 cdkl1 (Catalog #AAA6284544) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDKL1, CT (CDKL1, Cyclin-dependent kinase-like 1, Protein kinase p42 KKIALRE, Serine/threonine-protein kinase KKIALRE) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CDKL1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CDKL1 cdkl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDKL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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